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细胞外ATP影响铜离子诱导烟草悬浮细胞的死亡和H2O2的产生

白晶月 冯汉青 管冬冬 孙坤 贾凌云 李巧峡 张晋平

白晶月, 冯汉青, 管冬冬, 孙坤, 贾凌云, 李巧峡, 张晋平. 细胞外ATP影响铜离子诱导烟草悬浮细胞的死亡和H2O2的产生[J]. 华东师范大学学报(自然科学版), 2016, (3): 102-114. doi: 2016.03.012
引用本文: 白晶月, 冯汉青, 管冬冬, 孙坤, 贾凌云, 李巧峡, 张晋平. 细胞外ATP影响铜离子诱导烟草悬浮细胞的死亡和H2O2的产生[J]. 华东师范大学学报(自然科学版), 2016, (3): 102-114. doi: 2016.03.012
BAI Jing-Yue, FENG Han-Qing, GUAN Dong-Dong, SUN Kun, JIA Ling-Yun, LI Qiao-Xia, ZHANG Jin-Ping. Extracellular ATP affects the copper–induced cell death and H2O2 production in tobacco (Nicotiana tabacum L) cell-suspension cultures[J]. Journal of East China Normal University (Natural Sciences), 2016, (3): 102-114. doi: 2016.03.012
Citation: BAI Jing-Yue, FENG Han-Qing, GUAN Dong-Dong, SUN Kun, JIA Ling-Yun, LI Qiao-Xia, ZHANG Jin-Ping. Extracellular ATP affects the copper–induced cell death and H2O2 production in tobacco (Nicotiana tabacum L) cell-suspension cultures[J]. Journal of East China Normal University (Natural Sciences), 2016, (3): 102-114. doi: 2016.03.012

细胞外ATP影响铜离子诱导烟草悬浮细胞的死亡和H2O2的产生

doi: 2016.03.012
基金项目: 

国家自然科学基金(31260059, 30900105); 教育部科学技术研究重点项目(211190); 甘肃省财政厅高校基本科研业务项目; 甘肃科技计划(145RJYA258)

详细信息
    作者简介:

    白晶月, 女, 硕士研究生, 研究方向为逆境植物生理生态.

    通讯作者:

    贾凌云, 男, 博士研究生, 实验师, 研究方向为细胞与分子生物学

  • 中图分类号: Q945.78

Extracellular ATP affects the copper–induced cell death and H2O2 production in tobacco (Nicotiana tabacum L) cell-suspension cultures

  • 摘要: 细胞外ATP是植物体中许多生理过程的调节信号.胞外ATP可以影响铜离子诱导的细胞死亡和H_{2}O_{2}的产生.100sim 700 textit{mu }molL CuCl_{2}导致烟草悬浮细胞的死亡量显著上升,而且胞内H_{2}O_{2}和胞外H_{2}O_{2}的含量也随之上升.选择了300 textit{mu }molLCuCl_{2}研究铜离子导致的细胞死亡量上升和H_{2}O_{2}产生的过程.结果表明, 此浓度的 CuCl_{2}提升了NADPH氧化酶的活性,而加入DPI(NADPH氧化酶抑制剂)缓解了CuCl_{2}引起的细胞死亡和H_{2}O_{2}的产生,这说明CuCl_{2}引起细胞死亡和H_{2}O_{2}产生与NADPH氧化酶活性的增加相关. 加入50textit{mu }molLATP进一步增加了CuCl_{2}引起的细胞死亡、H_{2}O_{2}的产生、NADPH氧化酶.而DPI预处理后,外源ATP并未引起变化. 这一实验表明,胞外ATP可以通过刺激NADPH氧化酶影响铜离子引起的细胞活性和H_{2}O_{2}产生的变化
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出版历程
  • 收稿日期:  2015-06-19
  • 刊出日期:  2016-05-25

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