试验以酪蛋白为蛋白源，配制了维生素B6含量分别为0,10,30,60,100,150,200和400 mg/kg的8种等氮等能的半精制饲料，在静水系统中投喂体重为(2.12±0.56) g的幼蟹10周.结果表明，饲料中不同含量的维生素B6对中华绒螯蟹的存活率、增重率、体水分和灰分含量等均没有显著影响，但饲料中添加维生素B6能够显著提高幼蟹蛋白利用率、体粗蛋白含量和粗脂肪含量.饲料中维生素B6含量由0~100 mg/kg升高时，蛋白利用率随之显著提高.体粗蛋白和粗脂肪含量的变化趋势与蛋白质利用率相似，其中维生素B6添加量为100 mg/kg的处理组，幼蟹的粗蛋白和粗脂肪含量显著高于对照组和400 mg/kg维生素B6添加组.幼蟹肝胰腺中谷草转氨酶和谷丙转氨酶的活力先随着维生素B6添加量升高而增强，添加100 mg/kg维生素B6的试验组，幼蟹的谷丙转氨酶活力显著高于其它各试验组.根据各处理组中华绒螯蟹对饲料蛋白的利用率和肝胰腺谷丙转氨酶活力与饲料中维生素B6的关系，采用折线回归法计算得出中华绒螯蟹幼蟹对饲料中维生素B6的最适需求量为81.675~85.740 mg/kg饲料.
The basal diet was formulated using vitamin B6-free casein as the protein source. Eight graded levels (0, 10, 30, 60,100,150,200 and 400 mg vitamin B6 per kg diet) of vitamin B6 were added to the basal diet. Each diet was fed to three replicate groups of E.sinensis (2.12±0.56) g for 10 weeks. Results showed that there were no significant differences in survival, weight gain, and the content of water and ash among crabs fed different diets. But protein utilization ratio (PUR) increased significantly with the increase of dietary vitamin B6. The highest PUR was found in crabs fed diet with 100 mg vitamin B6 per kg diet. Patterns of the content of crude protein and crude fat were similar to that of PUR. Crabs fed 100 mg vitamin B6/kg diet had a significantly higher protein and fat content than that fed 0 and 400 mg vitamin B6 per kg diet. Besides, different dietary vitamin B6 content also had significant effect on the activities of hepatopancreas glutamate pyruvate transaminase (ALT) ,while no significant difference was observed in glutamate aspartate transaminase (AST). But they both increased with the increase of dietary vitamin B6. The maximum hepatopancreas ALT activitiy was observed in crab fed the 100 mg vitamin B6 per kg diet, and was significantly higher than all the other, except 30 mg vitamin B6 per kg diet groups. Combined, results in this study indicated that through stimulating the activity of transaminase, vitamin B6 could assist the anabolism of protein, and then improved the accumulation of body protein and fat in crabs. Broken-line regression analysis showed that the optimum dietary pyridoxine requirement for maximum PUR and hepatopancreas ALT activity of E. sinensis under these experimental conditions was 81.675-85.74 mg pyridoxine per kg diet.