中国综合性科技类核心期刊(北大核心)

中国科学引文数据库来源期刊(CSCD)

美国《化学文摘》(CA)收录

美国《数学评论》(MR)收录

俄罗斯《文摘杂志》收录

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

三丁基锡对热带爪蟾蝌蚪甲调基因mRNA表达的影响

曹庆珍 朱攀 袁静 张小利 刘俊奇 黄民生 施华宏

曹庆珍, 朱攀, 袁静, 张小利, 刘俊奇, 黄民生, 施华宏. 三丁基锡对热带爪蟾蝌蚪甲调基因mRNA表达的影响[J]. 华东师范大学学报(自然科学版), 2011, (6): 65-74.
引用本文: 曹庆珍, 朱攀, 袁静, 张小利, 刘俊奇, 黄民生, 施华宏. 三丁基锡对热带爪蟾蝌蚪甲调基因mRNA表达的影响[J]. 华东师范大学学报(自然科学版), 2011, (6): 65-74.
CAO Qing-zhen, ZHU Pan, YUAN Jing, ZHANG Xiao-li, LIU Jun-qi, HUANG Min-sheng, SHI Hua-hong. Effects of tributyltin on mRNA expressions of thyroid hormone response genes in Xenopus tropicalis tadpoles[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 65-74.
Citation: CAO Qing-zhen, ZHU Pan, YUAN Jing, ZHANG Xiao-li, LIU Jun-qi, HUANG Min-sheng, SHI Hua-hong. Effects of tributyltin on mRNA expressions of thyroid hormone response genes in Xenopus tropicalis tadpoles[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 65-74.

三丁基锡对热带爪蟾蝌蚪甲调基因mRNA表达的影响

详细信息
  • 中图分类号: Q948

Effects of tributyltin on mRNA expressions of thyroid hormone response genes in Xenopus tropicalis tadpoles

  • 摘要: 用三丁基锡(TBT)对热带爪蟾(Xenopus tropicalis)NF51阶段蝌蚪进行48 h和96 h暴露.结果显示,50 ngL-1和200 ngL-1 TBTCl暴露对胚胎的存活率没有影响.50 ngL-1 TBTCl暴露组TR和DI 3 mRNA在蝌蚪尾巴中的表达比对照组增加了1~2.3倍.200 ngL-1 TBTCl暴露组48 h时,TSH mRNA在蝌蚪尾巴中的表达比对照组上调了2.0倍.96 h后,TBTCl暴露组的RXR mRNA在脑和尾中的表达均比对照组减少了约0.4倍,而RXR mRNA在尾巴中的表达上调了5.9倍.96 h后,50 ngL-1 TBT暴露组MCT8 mRNA在尾部的表达上调了3.2倍,而200 ngL-1组在脑部的表达是对照组的0.23倍.PPAR mRNA在TBTCl暴露组蝌蚪脑和尾中的表达均比对照组增加了1~2倍.上述结果表明,TBT能引起蝌蚪多个甲调基因mRNA表达的变化,并具有明显的组织差异性.这些mRNA涉及到甲状腺激素的合成、运输、转化和作用等多个过程,表明TBT对爪蟾具有甲状腺激素干扰效应.同时,通过对一些相关mRNA的检测表明,TBT可能通过与维甲酸核受体(RXR)结合而对蝌蚪产生甲状腺激素干扰效应.由此可见,分子指标在筛选甲状腺激素干扰物的过程中不仅具有快速灵敏的特点,而且有助于解析污染物的致毒作用模式.
  • [1] [1]CHAMP M A. A review of organotin regulatory strategies, pending actions, related costs and benefits [J]. Sci Total Environ, 2000, 258: 21-71.

    [2]HALL L W. Tributyltin environmental studies [J]. Mar Pollut Bull, 1988, 19: 431-438.

    [3]CARLSSON G, KULKARNI P, LARSSON P, et al. Distribution of BDE-99 and effects on metamorphosis of BDE-99 and -47 after oral exposure in Xenopus tropicalis [J]. Aquat Toxicol, 2007, 84: 71-79.

    [4]RUDEL H, MULER J, STEINHANSES J, et al. Retrospective monitoring of organotin compounds in freshwater fish from 1988 to 2003: Results from the German environmental specimen bank [J]. Chemosphere, 2007, 66(10):1884-1894.

    [5]SHI H H, HUANG C J, YU X J, et al. Generalized system of imposex and reproductive failure of females in gastropods along the coastal waters of Mainland China [J]. Mar Ecol Prog Ser, 2005, 304: 179-189.

    [6]OEHIMANN J, BENEDETTO P D, TILLMANN M, et al. Endocrine disruption in prosobranch mollusks: evidence and ecological relevance [J]. Ecotoxicology, 2007, 16: 29-43.

    [7]王蕾, 刘青坡, 郭素珍, 等. TBT对非洲爪蟾甲状腺组织的影响 [J]. 华东师范大学学报:自然科学版, 2008(6): 75-81.

     WANG L, LIU Q P, GUO S Z, et al. Effects of tributyltin on thyroid histology of Xenopus laevis [J]. Journal of East China Normal University: Natural Science, 2008(6): 75-81.

    [8]SHI Y B, RITCHIE J W, TAYLOR P M. Complex regulation of thyroid hormone action: multiple opportunities for pharmacological intervention [J]. Pharmacol Therapeut, 2002, 94(3): 235-251.

    [9]KRAIN L P, DENVER R J. Developmental expression and hormonal regulation of glucocorticoid and thyroid hormone receptors during metamorphosis in Xenopus laevis [J]. Endocrinol, 2004, 181(1): 91-104.

    [10] JAGNYSCH O, OPITZ R, LUTZ I, et al. Effects of tetrabromobisphenol A on larval development and thyroid hormone-regulated biomarkers of the amphibian Xenopus laevis [J]. Environ Res, 2006, 101: 340-348.

    [11] HUANG H, CAI L, REMO B F, et al. Timing of metamorphosis and the onset of the negative feedback loop between the thyroid gland and the pituitary is controlled by type Ⅱ iodothyronine deiodinase in Xenopus laevis [J]. Proc Natl Acad Sci USA, 2001, 98(13): 7348-7353.

    [12] CAI L, BROWN D D. Expression of type II iodothyronine deiodinase marks the time that a tissue responds to thyroid hormone 2 induced metamorphosis in Xenopus laevis [J]. Dev Biol, 2004, 266(1): 87-95.

    [13] HELBING C C, BAILEY C M, JI L, et al. Identification of gene expression indicators for thyroid axis disruption in a Xenopus laevis metamorphosis screening assay. Part 1. Effects on the brain [J]. Aquat Toxicol, 2007, 82(4): 227-241.

    [14] OPITZ R, LUTZ I, NGUYEN N H, et al. Analysis of thyroid hormone receptor beta mRNA expression in Xenopus laevis tadpoles as a means to detect agonism and antagonism of thyroid hormone action [J]. Toxicol Appl Pharmacol, 2006, 212(1): 1-13.

    [15] 裘佳, 陈虹, 黄秉仁. 甲状腺激素受体在调节靶基因转录中的激活和抑制作用 [J]. 生命的化学, 2009, 29(3): 370-373.

     QIU J, CHEN H, HUANG B R. Transcriptional activation and repression regulation in target gene by thyroid hormone receptors [J]. Life Chem, 2009, 29(3): 370-373.

    [16] LI D, YAMADA T, WANG F, et al. Novel roles of retinoid X receptor (RXR) and RXR ligand in dynamically modulating the activity of the thyroid hormone receptor/RXR heterodimer [J]. Biol Chem, 2004, 279(9): 7427-7437.

    [17] NISHIKAWA J. Imposex in marine gastropods may be caused by binding of organotins to retinoid X receptor [J]. Mar Biol, 2006, 149: 117-124.

    [18] IWAMURO S, YAMADA M, KATO M, et al. Effects of bisphenol A on thyroid hormone-dependent up-regulation of thyroid hormone receptor α and β and down-regulation of retinoid X receptor γ in Xenopus tail culture[J]. Life Sci, 2006, 79: 2165-2171.

    [19] BORDJI K, GIRLLASEA J P, GOUZE J N, et al. Evidence for the presence of peroxisome proliferator-activated receptor (PPAR) α and γ and retinoid receptor in cartilage PPARγ activation modulates the effects of interleukin-1β on rat chondrocytes [J]. Biol Chem, 2000, 275: 12243-12250.

    [20] FRIESEMA E C, GANGULY S, ABDALLA A, et al. Identification of momocarboxylate transporter 8 as a specific thyroid hormone transporter [J]. Biol Chem, 2003, 278(41): 40128-40135.

    [21] FRIESEMA E C, KUIPER G G, JANSEN J, et al. Thyroid hormone transport by the human momocarboxylate transporter 8 and its rate-limiting role in intracellular metabolism [J]. Mol Endocrinol, 2006, 20(11): 2761-2772.
  • 加载中
计量
  • 文章访问数:  2543
  • HTML全文浏览量:  51
  • PDF下载量:  2211
  • 被引次数: 0
出版历程
  • 收稿日期:  2010-12-01
  • 修回日期:  2011-03-01
  • 刊出日期:  2011-11-25

目录

    /

    返回文章
    返回