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转化三七提取物为人参皂苷Rg3的真菌分离鉴定

王青 袁萍 茅仁刚 王菊勇 郑展

王青, 袁萍, 茅仁刚, 王菊勇, 郑展. 转化三七提取物为人参皂苷Rg3的真菌分离鉴定[J]. 华东师范大学学报(自然科学版), 2011, (6): 115-121,151.
引用本文: 王青, 袁萍, 茅仁刚, 王菊勇, 郑展. 转化三七提取物为人参皂苷Rg3的真菌分离鉴定[J]. 华东师范大学学报(自然科学版), 2011, (6): 115-121,151.
WANG Qing, YUAN Ping, MAO Ren-gang, WANG Ju-yong, ZHENG Zhan. Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg3[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 115-121,151.
Citation: WANG Qing, YUAN Ping, MAO Ren-gang, WANG Ju-yong, ZHENG Zhan. Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg3[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 115-121,151.

转化三七提取物为人参皂苷Rg3的真菌分离鉴定

详细信息
  • 中图分类号: Q93-33

Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg3

  • 摘要: 从培养人参(Panar ginseng C.A.Meyer)的土壤中筛选出23株菌,分别进行三七(Panax Notoginseng)提取物转化实验.经过摇瓶发酵后,采用TLC法与HPLC法对其发酵液提取物进行分析.研究结果表明,能够转化三七提取物为高活性人参皂苷的微生物菌株有9株,其中以菌株TR-20的转化作用最强.进一步对其发酵产物分析表明,菌株TR-20能够转化三七提取物为人参皂苷Rg3,转化产物中人参皂苷Rg3含量为190.2 mg/L,RSD为0.69%(n=5).通过对菌株TR-20的形态和培养特征观察,表明菌株TR-20属于棒曲霉属(Aspergillus sp).
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出版历程
  • 收稿日期:  2010-11-01
  • 修回日期:  2011-02-01
  • 刊出日期:  2011-11-25

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