Long-term potentiation of prefrontal cortex in NR1 knockout mice
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摘要: 用前脑特异性NR1基因敲除小鼠,采用离体脑片场电位技术,研究了NR1亚基在前额叶脑区突触可塑性中的作用.刺激强度反应(input-output curve)和双脉冲抑制反应(paired pulse depression, PPD)的结果表明,与同窝对照组小鼠相比,NR1基因敲除小鼠前额叶脑区的基本突触传递无明显变化.采用高频刺激(100 Hz, 1 000 ms 2, 间隔30 s)在小鼠的前额叶脑区诱导长时程增强(long-term potentiation, LTP),与对照组小鼠相比,NR1基因敲除小鼠前额叶脑区的LTP明显受损.以上数据提示,NR1亚基在前额叶脑区LTP的诱导中起着重要的作用.
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关键词:
- N-甲基-D-天门冬氨酸受体 /
- 前额叶皮层 /
- 长时程增强
Abstract: This study investigated the prefrontal synaptic plasticity of forebrain-specific NR1 knockout mice by using the vitro field potential recording technique. The results from the input-output and paired pulse depression curves suggest that the basal synaptic transmission is normal in the prefrontal cortex of NR1 knockout mice. Prefrontal long-term potentiation (LTP) induced by high frequency stimulation (two trains of 100 Hz with 1 s duration and 30 s interval) was abolished in slices of NR1 knockout mice. These results indicate that NR1 is critical in the induction of long-term potentiation in prefrontal cortex. -
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