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运用热带爪蟾胚胎检测黑臭河道底泥的生态毒性

杨波 朱攀 袁静 刘臻 黄民生 施华宏

杨波, 朱攀, 袁静, 刘臻, 黄民生, 施华宏. 运用热带爪蟾胚胎检测黑臭河道底泥的生态毒性[J]. 华东师范大学学报(自然科学版), 2012, (3): 145-153.
引用本文: 杨波, 朱攀, 袁静, 刘臻, 黄民生, 施华宏. 运用热带爪蟾胚胎检测黑臭河道底泥的生态毒性[J]. 华东师范大学学报(自然科学版), 2012, (3): 145-153.
YANG Bo, ZHU Pan, YUAN Jing, LIU Zhen, HUANG Min-sheng, SHI hua-hong. Screening the ecological toxicity of sediments in black-odors rivers using Xenopus tropicalis embryos[J]. Journal of East China Normal University (Natural Sciences), 2012, (3): 145-153.
Citation: YANG Bo, ZHU Pan, YUAN Jing, LIU Zhen, HUANG Min-sheng, SHI hua-hong. Screening the ecological toxicity of sediments in black-odors rivers using Xenopus tropicalis embryos[J]. Journal of East China Normal University (Natural Sciences), 2012, (3): 145-153.

运用热带爪蟾胚胎检测黑臭河道底泥的生态毒性

详细信息
  • 中图分类号: Q948

Screening the ecological toxicity of sediments in black-odors rivers using Xenopus tropicalis embryos

  • 摘要: 2010年9月~12月,在温州山下河(S)和九山外河(J)先后采集底泥样品5次.将热带爪蟾胚胎(NF10NF11)暴露于1〖DK〗∶4底泥提取液(第14次)和间隙水(第5次)中48 h,观测其发育状况.结果表明,山下河S1样点连续5次的底泥样品均导致胚胎全部死亡,表现出很强的毒性.暴露于山下河底泥提取液的胚胎体长均显著短于空白对照组,而暴露于九山外河底泥提取液的胚胎体长仅第3次显著短于空白对照组.暴露于山下河底泥提取液的胚胎畸形率均高于暴露于九山外河底泥提取液的,尤其在第4次检测中,暴露于S2-S4底泥提取液的胚胎畸形率均高达100%,而九山外河最高仅为70%(J2)和40%(J1).热带爪蟾胚胎直接暴露于所有采样点的山下河底泥间隙水均全部死亡.由此可见,山下河的整体毒性高于九山外河,底泥间隙水毒性高于底泥提取液.本研究表明,温州市典型的黑臭河道底泥对爪蟾胚胎的发育均有不同程度的影响,具有明显的生态毒性.同时,运用爪蟾胚胎可以有效地检测底泥的毒性效应,而且对不同河流和采样点的毒性评价具有较好的区分度.
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出版历程
  • 收稿日期:  2011-03-01
  • 修回日期:  2011-06-01
  • 刊出日期:  2012-05-25

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