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新型姜黄素结构衍生物抗肝癌细胞增殖作用的研究

李于博 文英 马明亮 吴良春 文珂 赵政

李于博, 文英, 马明亮, 吴良春, 文珂, 赵政. 新型姜黄素结构衍生物抗肝癌细胞增殖作用的研究[J]. 华东师范大学学报(自然科学版), 2012, (3): 161-170.
引用本文: 李于博, 文英, 马明亮, 吴良春, 文珂, 赵政. 新型姜黄素结构衍生物抗肝癌细胞增殖作用的研究[J]. 华东师范大学学报(自然科学版), 2012, (3): 161-170.
LI Yu-bo, WEN Ying, MA Ming-liang, WU Liang-chun, WEN Ke, ZHAO Zheng. Antiproliferative and apoptotic activities of novel curcumin analogs in human liver cancer cell lines[J]. Journal of East China Normal University (Natural Sciences), 2012, (3): 161-170.
Citation: LI Yu-bo, WEN Ying, MA Ming-liang, WU Liang-chun, WEN Ke, ZHAO Zheng. Antiproliferative and apoptotic activities of novel curcumin analogs in human liver cancer cell lines[J]. Journal of East China Normal University (Natural Sciences), 2012, (3): 161-170.

新型姜黄素结构衍生物抗肝癌细胞增殖作用的研究

详细信息
  • 中图分类号: R962

Antiproliferative and apoptotic activities of novel curcumin analogs in human liver cancer cell lines

  • 摘要: 采用MTT法对姜黄素结构衍生物(CCM系列化合物)进行抗人肝癌细胞Bel-7402和SMMC-7721活性筛选;利用流式细胞技术和荧光显微镜检测SMMC-7721细胞凋亡及周期分布;采用Western-Blot方法检测SMMC-7721中蛋白caspase-3和剪切后p17的表达.结果表明,化合物CCM-5和CCM-14抗肿瘤活性较好,其对SMMC-7721细胞的凋亡作用呈剂量依赖性,且凋亡率与阴性对照组相比有显著差异(P0.01);化合物浓度增高时,G0/G1期细胞减少,S期以及G2/M期细胞增加,凋亡峰SubG1峰增大;两个化合物均可增强caspase-3的表达,随着浓度的提高,caspase-3的表达趋势减弱,而剪切形式p17亚基表达量逐渐提高.因此,姜黄素结构衍生物CCM-5和CCM-14能抑制人肝癌细胞SMMC-7721细胞的增殖,促进凋亡,其作用机制可能与化合物诱导caspase-3活性的增强有关.
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  • 收稿日期:  2011-03-01
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  • 刊出日期:  2012-05-25

新型姜黄素结构衍生物抗肝癌细胞增殖作用的研究

  • 中图分类号: R962

摘要: 采用MTT法对姜黄素结构衍生物(CCM系列化合物)进行抗人肝癌细胞Bel-7402和SMMC-7721活性筛选;利用流式细胞技术和荧光显微镜检测SMMC-7721细胞凋亡及周期分布;采用Western-Blot方法检测SMMC-7721中蛋白caspase-3和剪切后p17的表达.结果表明,化合物CCM-5和CCM-14抗肿瘤活性较好,其对SMMC-7721细胞的凋亡作用呈剂量依赖性,且凋亡率与阴性对照组相比有显著差异(P0.01);化合物浓度增高时,G0/G1期细胞减少,S期以及G2/M期细胞增加,凋亡峰SubG1峰增大;两个化合物均可增强caspase-3的表达,随着浓度的提高,caspase-3的表达趋势减弱,而剪切形式p17亚基表达量逐渐提高.因此,姜黄素结构衍生物CCM-5和CCM-14能抑制人肝癌细胞SMMC-7721细胞的增殖,促进凋亡,其作用机制可能与化合物诱导caspase-3活性的增强有关.

English Abstract

李于博, 文英, 马明亮, 吴良春, 文珂, 赵政. 新型姜黄素结构衍生物抗肝癌细胞增殖作用的研究[J]. 华东师范大学学报(自然科学版), 2012, (3): 161-170.
引用本文: 李于博, 文英, 马明亮, 吴良春, 文珂, 赵政. 新型姜黄素结构衍生物抗肝癌细胞增殖作用的研究[J]. 华东师范大学学报(自然科学版), 2012, (3): 161-170.
LI Yu-bo, WEN Ying, MA Ming-liang, WU Liang-chun, WEN Ke, ZHAO Zheng. Antiproliferative and apoptotic activities of novel curcumin analogs in human liver cancer cell lines[J]. Journal of East China Normal University (Natural Sciences), 2012, (3): 161-170.
Citation: LI Yu-bo, WEN Ying, MA Ming-liang, WU Liang-chun, WEN Ke, ZHAO Zheng. Antiproliferative and apoptotic activities of novel curcumin analogs in human liver cancer cell lines[J]. Journal of East China Normal University (Natural Sciences), 2012, (3): 161-170.
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