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Mg2+对Bloom综合症解旋酶与G4DNA结合的影响研究

骆衡 蔡明娟 陈祥 丁玫 李坤 许厚强

骆衡, 蔡明娟, 陈祥, 丁玫, 李坤, 许厚强, . Mg2+对Bloom综合症解旋酶与G4DNA结合的影响研究[J]. 华东师范大学学报(自然科学版), 2012, (6): 81-88.
引用本文: 骆衡, 蔡明娟, 陈祥, 丁玫, 李坤, 许厚强, . Mg2+对Bloom综合症解旋酶与G4DNA结合的影响研究[J]. 华东师范大学学报(自然科学版), 2012, (6): 81-88.
LUO Heng, CAI Ming-juan, CHEN Xiang, DING Mei, LI Kun, XU Hou-qiang, . Study on the effects of Mg2+ on the binding between Bloom syndrome helicase and G4DNA[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 81-88.
Citation: LUO Heng, CAI Ming-juan, CHEN Xiang, DING Mei, LI Kun, XU Hou-qiang, . Study on the effects of Mg2+ on the binding between Bloom syndrome helicase and G4DNA[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 81-88.

Mg2+对Bloom综合症解旋酶与G4DNA结合的影响研究

详细信息
  • 中图分类号: R733.7

Study on the effects of Mg2+ on the binding between Bloom syndrome helicase and G4DNA

  • 摘要: 利用荧光偏振技术检测了Mg2+对G4DNA、BLM-G4DNA复合物和BLM642-1290解旋酶与G4DNA结合的影响.结果表明,G4DNA荧光偏振值随着Mg2+浓度的增加而增加(〖WTBX〗P〖WTBZ〗0.01);BLM-G4DNA复合物的荧光偏振值随着Mg2+浓度的增加出现下降升高下降的变化趋势(〖WTBX〗P〖WTBZ〗0.01);G4DNA与BLM642-1290解旋酶结合的荧光偏振值随着Mg2+浓度的增加而逐渐下降(〖WTBX〗P〖WTBZ〗0.01);分析不同Mg2+浓度下两种分子结合的〖WTBX〗Kd〖WTBZ〗值,发现Mg2+浓度为3.0 mmol/L时,BLM642-1290解旋酶与G4DNA最容易结合,表明适量Mg2+浓度会促进BLM642-1290与G4DNA的结合,但会引起两种分子结合的形状、流动性和电荷等性质的改变.这些结果可为进一步研究BLM解旋酶对G4DNA的作用机理提供相关资料.
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出版历程
  • 收稿日期:  2011-09-01
  • 修回日期:  2011-12-01
  • 刊出日期:  2012-11-25

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