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原花青素与戊二醛共交联心脏瓣膜的相容性

马旭兵 翟万银 张嘉敏 朱自严 张红锋 常江

马旭兵, 翟万银, 张嘉敏, 朱自严, 张红锋, 常江. 原花青素与戊二醛共交联心脏瓣膜的相容性[J]. 华东师范大学学报(自然科学版), 2013, (2): 92-102.
引用本文: 马旭兵, 翟万银, 张嘉敏, 朱自严, 张红锋, 常江. 原花青素与戊二醛共交联心脏瓣膜的相容性[J]. 华东师范大学学报(自然科学版), 2013, (2): 92-102.
MA Xu-bing, ZHAI Wan-yin, ZHANG Jia-min, ZHU Zi-yan, ZHANG Hong-feng, CHANG Jiang. Compatibility characteristics of acellular porcine heart valve matrix co-crosslinked with procyanidins and glutaraldehyde[J]. Journal of East China Normal University (Natural Sciences), 2013, (2): 92-102.
Citation: MA Xu-bing, ZHAI Wan-yin, ZHANG Jia-min, ZHU Zi-yan, ZHANG Hong-feng, CHANG Jiang. Compatibility characteristics of acellular porcine heart valve matrix co-crosslinked with procyanidins and glutaraldehyde[J]. Journal of East China Normal University (Natural Sciences), 2013, (2): 92-102.

原花青素与戊二醛共交联心脏瓣膜的相容性

详细信息
  • 中图分类号: Q819

Compatibility characteristics of acellular porcine heart valve matrix co-crosslinked with procyanidins and glutaraldehyde

  • 摘要: 采用8 mg/mL原花青素交联脱细胞猪主动脉心脏瓣膜4 h后,再经1.25 mg/mL戊二醛交联44 h.生物相容性检测结果显示,共交联瓣膜组细胞粘附率较戊二醛单独交联提高59%((78.758.7)%~(19.753.27)%)、无明显溶血现象((0.750.36)%)、血小板粘附量较戊二醛单独交联显著减少((19315.5)个 vs (292.624.93)个)、免疫原性低((49.336.3)% vs (95.275.26)%). 研究结果表明,共交联瓣膜材料有良好的细胞相容性、较好的血液相容性和较低的免疫原性,是一种优良的生物瓣材料,有望应用于生物瓣制备.
  • [1] [1] JAYAKRISHNAN A, JAMEELA S R. Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices[J]. Biomaterials, 1996, 17: 471-484.

    [2] SCHOEN F J, LEVY R J. Tissue heart valves: Current challenges and future research perspectives[J]. J Biomed Mater Res, 1999, 47: 439-465.

    [3] CHANG Y, HSU C K, WEI H J, et al. Cell-free xenogenic vascular grafts fixed with glutaraldehyde or genipin: In vitro and in vivo studies[J]. J Biotechnol, 2005, 120: 207-219.

    [4] ZHAI W, L X, CHANG J, et al. Quercetin-crosslinked porcine heart valve matrix: Mechanical properties, stability, anticalcification and cytocompatibility[J]. Acta Biomater, 2010(6): 389-395.

    [5] SCHMIDT C E,BAIER J M. Acellular vascular tissues: Natural biomaterials for tissue repair and tissue engineering[J]. Biomaterials, 2000, 21: 2215-2231.

    [6] ISENBURG J C, SIMIONESCU D T,VYAVAHARE N R. Tannic acid treatment enhances biostability and reduces calcification of glutaraldehyde fixed aortic wall[J]. Biomaterials, 2005, 26: 1237-1245.

    [7] NORBERT W G, HANGORG Z, HANS H S. Nano-coating with titanium of glutaraldehyde-fixed heart valve prostheses enables a reduced immune response and a self-seeding within circulation[G]//DANIEL E. Regenerative Medicine and Tissue Engineering-Cells and Biomaterials. Rijeka: InTech,2011.

    [8] ZHAI W, CHANG J, LIN K, et al. Crosslinking of decellularized porcine heart valve matrix by procyanidins[J]. Biomaterials, 2006, 27: 3684-3690.

    [9] ZHAI W, CHANG J, L X, et al. Procyanidins-crosslinked heart valve matrix: Anticalcification effect[J]. J Biomed Mater Res B, 2009, 90B: 913-921.

    [10] FURIGA A, LONVAUD F, BADET C. In vitro study of antioxidant capacity and antibacterial activity on oral anaerobes of a grape seed extract[J]. Food Chem, 2009, 113: 1037-1040.

    [11] SIVAKUMARAN S, MOLAN A L, MEAGHER L P, et al. Variation in antimicrobial action of proanthocyanidins from Dorycnium rectum against rumen bacteria[J]. Phytochemistry, 2004, 65: 2485-2497. 

    [12] SANO T, ODA E, YAMASHITA T, et al. Anti-thrombotic effect of proanthocyanidin, a purified ingredient of grape seed[J]. Thromb Res, 2005, 115: 115-121.

    [13] LIU Y Z, CAO Y G, YE J Q, et al. Immunomodulatory effects of proanthocyanidin A-1 derived in vitro from Rhododendron spiciferum[J]. Fitoterapia, 2010, 81: 108-114.

    [14] JOHNSON C M, HANSON M N,HELGESON S C. Porcine cardiac valvular subendothelial cells in culture-cell isolation and growth-characteristics[J]. J Mol Cell Cardiol, 1987, 19: 1185-1193.

    [15] BRODBECK W G, NAKAYAMA Y, MATSUDA T, et al. Biomaterial surface chemistry dictates adherent monocyte/macrophage cytokine expression in vitro[J]. Cytokine, 2002, 18: 311-319.

    [16] 李相仕. 壳聚糖基生物材料与巨噬细胞相互作用研究[D]. 天津:天津大学,2007.

    [17] BLAZSO G, GABOR M,ROHDEWALD P. Antiinflammatory activities of procyanidin-containing extracts from Pinus pinaster Ait after oral and cutaneous application[J]. Pharmazie, 1997, 52: 380-382. 

    [18] 刘兴光,汪钢,俞世强,等.组织工程心脏瓣膜支架材料的血液相容性[J].中国临床康复,2004,8(30):6659-6661.

    [19] ZHOU J Y, FRITZE O, SCHLEICHER M, et al. Impact of heart valve decellularization on 3-D ultrastructure, immunogenicity and thrombogenicity[J]. Biomaterials, 2010, 31: 2549-2554.

    [20] OKAMURA K, CHIBA C, IRIYAMA T, et al. Antigen depressant effect of glutaraldehyde for aortic heterografts with a valve, with special reference to a concentration right fit for the preservation of grafts[J]. Surgery, 1980, 87: 170-176.

    [21] SIMON P, KASIMIR M T, SEEBACHER G, et al. Early failure of the tissue engineered porcine heart valve SYNERGRAFT (TM) in pediatric patients[J]. Eur J Cardio-Thorac Sur, 2003, 23: 1002-1006.
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出版历程
  • 收稿日期:  2012-02-01
  • 修回日期:  2012-05-01
  • 刊出日期:  2013-03-25

原花青素与戊二醛共交联心脏瓣膜的相容性

  • 中图分类号: Q819

摘要: 采用8 mg/mL原花青素交联脱细胞猪主动脉心脏瓣膜4 h后,再经1.25 mg/mL戊二醛交联44 h.生物相容性检测结果显示,共交联瓣膜组细胞粘附率较戊二醛单独交联提高59%((78.758.7)%~(19.753.27)%)、无明显溶血现象((0.750.36)%)、血小板粘附量较戊二醛单独交联显著减少((19315.5)个 vs (292.624.93)个)、免疫原性低((49.336.3)% vs (95.275.26)%). 研究结果表明,共交联瓣膜材料有良好的细胞相容性、较好的血液相容性和较低的免疫原性,是一种优良的生物瓣材料,有望应用于生物瓣制备.

English Abstract

马旭兵, 翟万银, 张嘉敏, 朱自严, 张红锋, 常江. 原花青素与戊二醛共交联心脏瓣膜的相容性[J]. 华东师范大学学报(自然科学版), 2013, (2): 92-102.
引用本文: 马旭兵, 翟万银, 张嘉敏, 朱自严, 张红锋, 常江. 原花青素与戊二醛共交联心脏瓣膜的相容性[J]. 华东师范大学学报(自然科学版), 2013, (2): 92-102.
MA Xu-bing, ZHAI Wan-yin, ZHANG Jia-min, ZHU Zi-yan, ZHANG Hong-feng, CHANG Jiang. Compatibility characteristics of acellular porcine heart valve matrix co-crosslinked with procyanidins and glutaraldehyde[J]. Journal of East China Normal University (Natural Sciences), 2013, (2): 92-102.
Citation: MA Xu-bing, ZHAI Wan-yin, ZHANG Jia-min, ZHU Zi-yan, ZHANG Hong-feng, CHANG Jiang. Compatibility characteristics of acellular porcine heart valve matrix co-crosslinked with procyanidins and glutaraldehyde[J]. Journal of East China Normal University (Natural Sciences), 2013, (2): 92-102.
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