PC交联脱细胞牛心包膜的力学性能、稳定性和血液相容性

马兵; 翟万银; 张嘉敏; 孙晓宁; 朱自严; 张红锋; 常江

PC交联脱细胞牛心包膜的力学性能、稳定性和血液相容性

1.
华东师范大学生命科学学院,上海 200062;
2.
中国科学院上海硅酸盐研究所生物材料与组织工程中心,上海 200050;
3.
上海市血液中心上海市输血研究所,上海 200051;
4.
复旦大学附属中山医院心脏外科,上海 200032

详细信息

中图分类号: Q33
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出版历程
- 收稿日期: 2012-07-01
- 修回日期: 2012-10-01
- 刊出日期: 2013-09-25

Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility

1.
School of Life Sciences, East China Normal University, Shanghai 200062, China;
2.
Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China;
3.
Shanghai Blood Center, Shanghai Institute of Blood Transfusion, Shanghai 200051, China;
4.
Department of Cardiac Surgery, Zhongshan Hospital Fudan University, Shanghai 200032, China

摘要

摘要: 测定了原花青素（PC）交联的脱细胞牛心包膜（dbpECM）的力学性能、稳定性和血液相容性.实验结果显示，2.5 mg/mL原花青素交联能将拉伸强度从29.1 MPa提高到51.3 MPa，并保持弹性模量基本不变，而抗胶原酶降解能力与戊二醛相当；PC-dbpECM浸泡于D-Hanks溶液中，仅过量未交联原花青素在一周内释放，而交联的PC在PC-dbpECM中保持稳定.1 mg/mL PC即可基本抑制体外钙化.PC或GA交联的dbpECM都有较好的血液相容性，但PC交联明显优于GA. 2.5 mg/mL PC交联的dbpECM血小板的粘附率（7.8%）显著低于未交联组（26.1%）和GA交联组（36.6%），表明PC-dbpECM具有较好的抗血栓形成能力.结果表明PC-dbpECM具有良好的力学性能，稳定性和血液相容性，有可能成为制备人工心脏瓣膜的优良材料.
- 原花青素 /
- 脱细胞牛心包膜 /
- 力学性能 /
- 稳定性 /
- 血液相容性
Abstract: In this study, decellularized bovine pericardium matrix (dbpECM) was cross-linked by using PC at series concentrations. Mechanical properties, stability and hemo-compatibility of PC-crosslinked dbpECM (PC-dbpECM) were investigated. The results show that the tensile strength of the PC-dbpECM which crosslinked by the 2.5 mg/mL PC is increased from 29.1 MPa to 51.3 MPa with the elastic modulus roughly at basic level. Resistance of PC-dbpECM to collagenase hydrolysis is equivalent to that of GA-crosslinked. When PC-dbpECM soaked in D-Hanks solution, only the excessive non-crosslinked PC released within a week, leaving the crosslinked PC stable all the experiment period. PC crosslinked dbpECM (1 mg/mL) can effectively inhibit calcification in vitro. Both PC- and GA-dbpECM have ideal hemo-compatibility, but the PC cross-linking is superior to that of GA. The rate of platelet adhesion on the surface of 2.5 mg/mL PC crosslinked dbpECM (7.8%) was significantly lower than that of non-crosslinked group (26.1%) and the GA crosslinked group (36.6%), indicating that PC-dbpECM has low coagulation spotential. These data suggest that PC-dbpECM has good mechanical properties, stability and hemo-compatibility, suitable for preparing biological heart valves.
- procyanidins /
- decellularized bovine pericardium /
- mechanical properties /
- stability /
- hemo-compatibility

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参考文献(1)

[1]

［1］ JAYAKRISHNAN A, JAMEELA S R. Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices［J］.Biomaterials, 1996, 17(5):471-484.

［2］ SCHMIDT C E, BAIER J M. Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering［J］. Biomaterials, 2000, 21(22):2215-2231.

［3］ STOCK U A, SCHENKE L K. Performance of decellularized xenogeneic tissue in heart valve replacement［J］. Biomaterials, 2006 , 27(1):1-2.

［4］ MANJI R A, ZHU L F, NIJIAR N K, et al.Glutaraldehyde-fixed bioprosthetic heart valve conduits calcify and fail from xenograft rejection［J］. Circulation, 2006, 114(4):318-327.

［5］ ELLSMERE J C, KHANNA R A, LEE J M. Mechanical loading of bovine pericardium accelerates enzymatic degradation［J］. Biomaterials, 1999, 20(12):1143-1150.

［6］ LEE C, KIM S H, CHOI S H, et al. High-concentration glutaraldehyde fixation of bovine pericardium in organic solvent and post-fixation glycine treatment: in vitro material assessment and in vivo anticalcification effect［J］. Eur J Cardiothorac Surg, 2011, 39(3):381-387. 

［7］ ISENBURY J C, SIMIONESCU D T, VYAVAHARE N R.Tannic acid treatment enhances biostability and reduces calcification of glutaraldehyde fixed aortic wall［J］. Biomaterials, 2005, 26(11):1237-1245. 

［8］ CHANDA J, KURIBAYASHI R, ABE T. Use of the glutaraldehyde-chitosan-treated porcine pericardium as a pericardial substitute［J］. Biomaterials, 1996, 17(11):1087-1091. 

［9］ GUYOT S, MARNET N, SANONER P, et al. Variability of the polyphenolic composition of cider apple (Malus domestica) fruits and juices［J］. J Agric Food Chem, 2003, 51(21): 6240-6247. 

［10］ YAMAKOSHI J, SAITO M, KATAOKA S, et al. Safety evaluation of proanthocyanidin-rich extract from grape seeds［J］. Food Chem Toxicol, 2002, 40(5):599-607.

［11］ PACKER L, RIMBACH G, VIRGILI F. Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritima) bark, pycnogenol［J］. Free Radic Biol Med, 1999, 27(5-6):704-724.

［12］ ZHAI W, JIA C, ZHAO H, et al. Procyanidins inhibit tumor angiogenesis by crosslinking extracellular matrix［J］. Chinese J Cancer Res, 2011, 23 (2):99-106.

［13］ ZHAI W, CHANG J, LIN K, et al. Crosslinking of decellularized porcine heart valve matrix by procyanidins［J］. Biomaterials, 2006, 27(19): 3684-3690. 

［14］ HAN B, JAUREQUI J, TANG B W, et al. Proanthocyanidin: A natural crosslinking reagent for stabilizing collagen matrices［J］. J Biomed Mater Res A, 2003, 65(1): 118-124.

［15］ ZHAI W, CHANG J, LU X, et al. Procyanidins-crosslinked heart valve matrix: anti-calcification effect［J］. J Biomed Mater Res B Appl Biomater, 2009, 90(2):913-921.

［16］ SCHOEN F J, LEVY R J. Tissue heart valves: current challenges and future research perspectives［J］. J Biomed Mater Res, 1999, 47(4):439-465.

［17］ VESELY I. Heart valve tissue engineering［J］. Circ Res, 2005, 97(8):743-755.

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