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EcR-RNAi和印楝素处理斜纹夜蛾幼虫诱导腹足发育受阻表型

赵锦程 吴天旻 刘丽花 汪洋 何琳

赵锦程, 吴天旻, 刘丽花, 汪洋, 何琳. EcR-RNAi和印楝素处理斜纹夜蛾幼虫诱导腹足发育受阻表型[J]. 华东师范大学学报(自然科学版), 2014, (1): 133-142.
引用本文: 赵锦程, 吴天旻, 刘丽花, 汪洋, 何琳. EcR-RNAi和印楝素处理斜纹夜蛾幼虫诱导腹足发育受阻表型[J]. 华东师范大学学报(自然科学版), 2014, (1): 133-142.
ZHAO Jin-cheng, WU Tian-min, LIU Li-hua, WANG Yang, HE Lin. EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.[J]. Journal of East China Normal University (Natural Sciences), 2014, (1): 133-142.
Citation: ZHAO Jin-cheng, WU Tian-min, LIU Li-hua, WANG Yang, HE Lin. EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.[J]. Journal of East China Normal University (Natural Sciences), 2014, (1): 133-142.

EcR-RNAi和印楝素处理斜纹夜蛾幼虫诱导腹足发育受阻表型

详细信息
  • 中图分类号: Q965

EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.

  • 摘要: 蜕皮激素是一种可以诱导一系列变态发育关键过程的昆虫蜕皮调节激素, 与蜕皮激素受体结合以后与配体蛋白~Usp~作用形成一个复合体发挥作用. 通过兼并引物克隆获得斜纹夜蛾一个~600~bp~的~EcR cDNAs~片段编码~200~个氨基酸, 斜纹夜蛾是东亚地区一种主要的农作物害虫. 利用这段序列设计了~EcR-dsRNA~通过注射幼虫的实验来分析~EcR~基因在不同发育阶段的功能. 同时利用印楝素喂食RNAi注射的幼虫观察其与正常对照组有何发育差异. 结果显示~EcR RNAi~会影响正常的蜕皮过程并导致不完成蛹化, 在~EcR RNAi~注射并喂食印楝素的实验组, 幼虫出现生长迟缓、发育进程紊乱和腹足发育不正常等现象, 由此推测印楝素和~EcR RNAi~或许影响了蜕皮级联调控通路, 由此引发变态发育过程中组织重建紊乱.
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    during Drosophila development [J]. Mol Cell, 2011, 44(1):51-61.
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    \textit{Spodoptera litura}[J]. Ann N Y Acad Sci, 2005, 1040: 417-419.
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    {9} JIANHUA H, YONG Z, MINGHUI L, et al. RNA interference-mediated silencing of the bursicon gene induces defects in wing expansion [J]. FEBS Letters, 2007, 581: 697-701.
    {10} RAJAGOPAL, SIVAKUMAR S, AGRAWAL N, et al. Silencing of midgut aminopeptidase N of \textit{Spodoptera litura }by double-stranded RNA establishes its role as \textit{Bacillus thuringiensis }toxin receptor[J]. The Journal of Biological Chemistry,2002, 277(49): 46849-46851;
    {11} TARLOCHAN S D, GLENN R C and DAT P L. New insecticides with ecdysteroidal and juvenile hormone activity [J]. Annu Rev Entomol,1998, 43:545-69.
    {12} ALASDAIR J NISBET, A. JENNIFER MORDUE (LUNTZ), ROBERT B. GROSSMAN et al. Characterization of Azadirachtin Binding to Sf9 Nuclei In Vitro[J]. Archives of Insect Biochemistry and Physiology, 2001, 46:78-86.
    {13} MANAL M.ADEL, FRANTISEK SEHNAL. Azadirachtin potentiates the action of ecdysteroid agonist RH-2485 in\textit{ Spodoptera littoralis}[J]. Journal of Insect Physiology, 2000, 46:267-274.
    {14} MAREK ORLOWSKI, MONIKA SZYSZKA, AGNIESZKA KOWALSKA et al. Plasticity of the Ecdysone Receptor DNA Binding Domain[J]. Molecular Endocrinology, 2004, 18(9): 2166-2184.
    {15} DEVARAKONDA S, HARP JM, KIM Y, OZYHAR A, RASTINEJAD F. Structure of the heterodimeric ecdysone receptor DNA-binding complex[J]. EMBO J, 2003, 22: 5827-5840.
    {16} DAVID MARTIN, OSCAR MAESTRO, JOSEFA CRUZ et al. RNAi studies reveal a conserved role for RXR in molting in the cockroach \textit{Blattella germanica}[J]. Journal of Insect Physiology, 2006, 52(4): 410-416.
    {17} S.BANERJEE, H.REMBOLD. Azadirachtin a interferes with control of serotonin pools in the Neuroendocrine System of Locusts[J]. Naturwissenschaften, 1992, 79:81-84.
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    {19} TRUMAN, J. W. Neuroendocrine control of ecdysis [G]//OHNISHI E, ISHIZAKI H. Molting and Metamorphosis: In commemoration of the fiftieth anniversary of Soichi Fukuda's discovery of prothoracic gland function. Tokyo Berlin New York: Japan Scientific Societies Press, Springer-Verlag, 1990: 67-82.
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出版历程
  • 收稿日期:  2013-01-01
  • 修回日期:  2013-04-01
  • 刊出日期:  2014-01-25

EcR-RNAi和印楝素处理斜纹夜蛾幼虫诱导腹足发育受阻表型

  • 中图分类号: Q965

摘要: 蜕皮激素是一种可以诱导一系列变态发育关键过程的昆虫蜕皮调节激素, 与蜕皮激素受体结合以后与配体蛋白~Usp~作用形成一个复合体发挥作用. 通过兼并引物克隆获得斜纹夜蛾一个~600~bp~的~EcR cDNAs~片段编码~200~个氨基酸, 斜纹夜蛾是东亚地区一种主要的农作物害虫. 利用这段序列设计了~EcR-dsRNA~通过注射幼虫的实验来分析~EcR~基因在不同发育阶段的功能. 同时利用印楝素喂食RNAi注射的幼虫观察其与正常对照组有何发育差异. 结果显示~EcR RNAi~会影响正常的蜕皮过程并导致不完成蛹化, 在~EcR RNAi~注射并喂食印楝素的实验组, 幼虫出现生长迟缓、发育进程紊乱和腹足发育不正常等现象, 由此推测印楝素和~EcR RNAi~或许影响了蜕皮级联调控通路, 由此引发变态发育过程中组织重建紊乱.

English Abstract

赵锦程, 吴天旻, 刘丽花, 汪洋, 何琳. EcR-RNAi和印楝素处理斜纹夜蛾幼虫诱导腹足发育受阻表型[J]. 华东师范大学学报(自然科学版), 2014, (1): 133-142.
引用本文: 赵锦程, 吴天旻, 刘丽花, 汪洋, 何琳. EcR-RNAi和印楝素处理斜纹夜蛾幼虫诱导腹足发育受阻表型[J]. 华东师范大学学报(自然科学版), 2014, (1): 133-142.
ZHAO Jin-cheng, WU Tian-min, LIU Li-hua, WANG Yang, HE Lin. EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.[J]. Journal of East China Normal University (Natural Sciences), 2014, (1): 133-142.
Citation: ZHAO Jin-cheng, WU Tian-min, LIU Li-hua, WANG Yang, HE Lin. EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.[J]. Journal of East China Normal University (Natural Sciences), 2014, (1): 133-142.
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