Effects of tributyltin on mRNA expressions of thyroid hormone response genes in <em>Xenopus tropicalis</em> tadpoles

CAO Qing-zhen; ZHU Pan; YUAN Jing; ZHANG Xiao-li; LIU Jun-qi; HUANG Min-sheng; SHI Hua-hong

Issue 6

Nov. 2011

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2011 > (6): 65-74

CAO Qing-zhen, ZHU Pan, YUAN Jing, ZHANG Xiao-li, LIU Jun-qi, HUANG Min-sheng, SHI Hua-hong. Effects of tributyltin on mRNA expressions of thyroid hormone response genes in Xenopus tropicalis tadpoles[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 65-74.

Citation:

CAO Qing-zhen, ZHU Pan, YUAN Jing, ZHANG Xiao-li, LIU Jun-qi, HUANG Min-sheng, SHI Hua-hong. Effects of tributyltin on mRNA expressions of thyroid hormone response genes in Xenopus tropicalis tadpoles[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 65-74.

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Effects of tributyltin on mRNA expressions of thyroid hormone response genes in Xenopus tropicalis tadpoles

1.
Shanghai Key Laboratory of Urbanization Processes and Ecological Restoration, Department of Environment Science, East China Normal University, Shanghai 200062, China

Received Date: 2010-12-01
Rev Recd Date: 2011-03-01
Publish Date: 2011-11-25

Abstract

Abstract

Xenopus tropicalis tadpoles (NF51) were exposed to TBT for 48 and 96 h, respectively. Exposures to 50 and 200 ngL-1 TBTCl showed no effects on the survival rate of tadpoles. The expression of TR and DI3 mRNA were significantly increased in the tails by 1～2.3 fold in the groups treated with 50 ngL-1 TBTCl. The expression of TSH mRNA was increased in the tails by 2 fold after 48 h of exposure. RXR mRNA was decreased by 0.4 fold in the brains and tails, and RXR mRNA was increased by 5.9 fold in the tails after 96 h of exposure. MCT8 mRNA was increased by nearly 3 fold in the tails in 50 ngL-1 TBT treatment group and decreased by 3 fold or so in the brains in 50 ngL-1 TBT treatment group after 96 h of exposure. These results suggest that TBT can result in the changes of multiple mRNA expressions and show high tissue-specific differences. These mRNA are involved in the synthesis, transportations, transformations and actions, which indicate that TBT shows thyroid hormone disrupting effects. The changes of related mRNA expressions indicate that TBT might disrupt the thyroid hormone system of tadpoles by binding with retinoid X receptor. Therefore, biomarkers are not only cost-effective and sensitive endpoints in screening the thyroid disrupting chemicals but also useful in identifying the mode of action of contaminants.
- Xenopus tropicalis,
- TBT,
- thyroid hormone response genes expressions

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References(1)

References

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