中国综合性科技类核心期刊(北大核心)

中国科学引文数据库来源期刊(CSCD)

美国《化学文摘》(CA)收录

美国《数学评论》(MR)收录

俄罗斯《文摘杂志》收录

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Issue 6
Jan.  2013
Turn off MathJax
Article Contents
WU Jin, DONG Su-zhen. Establishment of GPR41 stable cell line and agonist screening of GPR41 receptor[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 73-80,102.
Citation: WU Jin, DONG Su-zhen. Establishment of GPR41 stable cell line and agonist screening of GPR41 receptor[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 73-80,102.

Establishment of GPR41 stable cell line and agonist screening of GPR41 receptor

  • Received Date: 2011-11-01
  • Rev Recd Date: 2012-02-01
  • Publish Date: 2012-11-25
  • In this study, a stable GPR41 receptor cell model was established. The GPR41 expression was detected by RT-PCR and western blot,while the function of GPR41 was confirmed by cAMP and Ca assays. These results have shown that we have successfully established GPR41 cell line which can be used for screening the agonists of the receptor in vitro. GPR41 receptor binding activity was tested by cAMP assay using secondary metabolites extracted from gulf seaweed aflatoxin c-f-3 in Putian Fujian. The results have also shown that the No.37 compound, a new compound belonging to 2-Pyrones, has GPR41 receptor agonist activity with high affinity. This is the first report on 2-Pyrones with GPR41 receptor agonist activity.
  • loading
  • [1]
    [1] BROWN A J, JUPE S, BRISCOE C P. A family of fatty acid binding receptors[J]. DNA Cell Biol, 2005, 24(1): 54-61.

    [2] LE POUL E, LOISON C, STRUYF S, et al. Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation[J]. J Biol Chem, 2003, 278(28): 25481-25489.

    [3] BROWN A J, GOLDSWORTHY S M, BARNES A A, et al. The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids[J]. J Biol Chem, 2003, 278(13): 11312-11319.

    [4] XIONG Y, MIYAMOTO N, SHIBATA K, et al. Short-chain fatty acids stimulate leptin production in adipocytes through the G protein-coupled receptor GPR41[J]. Proc Natl Acad Sci USA, 2004, 101(4): 1045-1050.

    [5] DREYFUSS M MCHAPELA I H. Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticals[J]. Biotechnology, 1994, 26: 49-80.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索
    Article views (2516) PDF downloads(2157) Cited by()
    Proportional views

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return