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WU Jin, DONG Su-zhen. Establishment of GPR41 stable cell line and agonist screening of GPR41 receptor[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 73-80,102.
Citation:
WU Jin, DONG Su-zhen. Establishment of GPR41 stable cell line and agonist screening of GPR41 receptor[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 73-80,102.
WU Jin, DONG Su-zhen. Establishment of GPR41 stable cell line and agonist screening of GPR41 receptor[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 73-80,102.
Citation:
WU Jin, DONG Su-zhen. Establishment of GPR41 stable cell line and agonist screening of GPR41 receptor[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 73-80,102.
Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key Laboratory of Brain Functional Genomics, East China Normal University, Shanghai 200062, China
In this study, a stable GPR41 receptor cell model was established. The GPR41 expression was detected by RT-PCR and western blot,while the function of GPR41 was confirmed by cAMP and Ca assays. These results have shown that we have successfully established GPR41 cell line which can be used for screening the agonists of the receptor in vitro. GPR41 receptor binding activity was tested by cAMP assay using secondary metabolites extracted from gulf seaweed aflatoxin c-f-3 in Putian Fujian. The results have also shown that the No.37 compound, a new compound belonging to 2-Pyrones, has GPR41 receptor agonist activity with high affinity. This is the first report on 2-Pyrones with GPR41 receptor agonist activity.
[1] BROWN A J, JUPE S, BRISCOE C P. A family of fatty acid binding receptors[J]. DNA Cell Biol, 2005, 24(1): 54-61.[2] LE POUL E, LOISON C, STRUYF S, et al. Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation[J]. J Biol Chem, 2003, 278(28): 25481-25489.[3] BROWN A J, GOLDSWORTHY S M, BARNES A A, et al. The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids[J]. J Biol Chem, 2003, 278(13): 11312-11319.[4] XIONG Y, MIYAMOTO N, SHIBATA K, et al. Short-chain fatty acids stimulate leptin production in adipocytes through the G protein-coupled receptor GPR41[J]. Proc Natl Acad Sci USA, 2004, 101(4): 1045-1050.[5] DREYFUSS M MCHAPELA I H. Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticals[J]. Biotechnology, 1994, 26: 49-80.