葡萄籽原花青素提取物抗氧化作用研究
Study on the Antioxidation Effects of Grape Seed Procyanidins Extract
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摘要: 为研究葡萄籽原花青素提取物(grape procyanidins extract,GPE)抗氧化和对DNA氧化损伤的保护作用,我们采用七种生物化学发光体系,检测了GPE 对O2-、OH、H2O2、ONOO-和全血嗜中性白细胞“呼吸爆发”产生的多种活性氧的清除作用,以及对OH引起的DNA氧化损伤的保护作用;采用Fe2+诱发脂蛋白多不饱和脂肪酸过氧化比色体系,检测了GPE对脂质过氧化分抑制作用。结果显示,GPE 能有效地清除O2-、OH、H2O2、ONOO-和全血嗜中性白细胞“呼吸爆发”产生的多种活性氧而抑制体系 发光,并有效抑制脂质过氧化,半抑制浓度分别约为0.2ug/ml、90ug/ml、0.5ug/ml、10ug/ml、130ug/ml、70ug/ml;25ug/ml的GPE对DNA氧化损伤的抑制率誉为70%。提示GPE 能有效清除多种自由基,保护DNA免受·OH引起的氧化损伤,嗜良好的抗氧化剂。Abstract: In order to study the antioxidation effects of grape seed procyanidins extract (GPE), seven chemiluminescence systems were used to determine the protective effect of GPE on DNA damage caused by ·OH and the scavenging effect of GPE on O2-、OH、H2O2 and ONOO- , and various reactive oxygen species produced during "respiration burst" of whole blood phagocytes. The inhibiting effect of GPE on lipid peroxidation was evaluated by using the colorimetric system of lipoprotein polyunsaturated fatty acids peroxidation induced by Fe2+. The experiments showed that GPE can effectively scavenge O2-、OH、H2O2 ,ONOO- and various reactive oxygen species prouduced during "respiration burst" of whole blood phagocytes, and inhibit lipid peroxidation (IC50s are 0.2ug/ml、90ug/ml、0.5ug/ml、10ug/ml、130ug/ml、70ug/ml respectively); the inhibiting rate of DNA oxidative damage is approximately 70%, when GPE at a concentration of 25ug/ml is given. These results indicate that GPE os an efficient antioxidant and can effectively scavenge various free radicals and provide effective protection anfinst DNA damage caused by ·OH.
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