Abstract: Cells of Dictyostelium discoideum are trigged to undergo development when they are depleted their food source. Cell adhesion molecules play an important role in maintaining the integrity of cell aggregation and influencing subsequent cell-type differentiation and cell sorting. At the loose aggregation stage , gp150 was localized on the peripheral area of multicellular aggregation.At the slug stage, the prespore cell domain and prestalk cell domain were separated by the gp150 molecule, quantity of the gp150 molecule become to increase in the pretalk cell domain following Dictyostelium discoideum development. Thus the temporal of gp150 molecule expression and the special localization of gp150 molecule are related with the differentiation of stalk cells Dictyostelium discoideum development.