Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis(Chinese)
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摘要: 通过二维电泳（2-DE）技术来研究脉冲电场作用后48 h中国红豆杉细胞在蛋白表达上的变化.共进行了3次二维电泳重复实验，经过相关因子分析证实具有较高的重复性.比较对照组和经过脉冲电场处理的实验组的二维电泳图谱，发现有较明显的差异.用基质辅助激光解析-飞行时间质谱和液相色谱-电喷雾质谱对其中的部分蛋白进行分析鉴定.鉴定结果表明：核糖核酸蛋白粒、醛糖差向异构酶和铁氧还蛋白-NADP(+)还原酶这三个蛋白可能是与脉冲电场诱导相关的蛋白.
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关键词:
- 脉冲电场 /
- 中国红豆杉细胞 /
- 二维电泳 /
- 基质辅助激光解析-飞行时间质谱 /
- 液相色谱-电喷雾质谱
Abstract: Two dimensional gel electrophoresis(2-DE) was used to analyse changes of the protein expression of suspension cultures of Taxus chinensis 48 hours after pulsed electric field (PEF) elicitation. The 2-DE experiments were repeated for 3 times, and proved to be reliable according to the correlation coefficient analysis. Distinct changes were found from comparison of the protein patterns of control and PEF treated cells. Matrixassisted laser desorption/ionization mass spectrometry and electrospray ionization mass spectrometry were used to analyse some proteins. The results indicated that heterogeneous nuclear ribonucleoprotein(hnRNP), aldose l-epimerase family protein and Ferredoxin-NADP(+) reductase, putative/adrenodoxin reductase are related to the induction of PEF. -
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