Cloning and expression pattern analysis of a cDNA of pkc-2gene in Caenorhabditis elegans (Chinese, Notes)
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摘要: 蛋白激酶C在秀丽小杆线虫中具有调节肌细胞渐进性萎缩的功能.为了揭示它的调节机制, 本研究克隆了秀丽小杆线虫中蛋白激酶C pkc-2基因的cDNA pkc-2-c，构建了含该pkc-2基因cDNA亚型的重组质粒pPD 118.20-pKG 63；揭示了该cDNA在秀丽小杆线虫体壁肌细胞中的定位.Abstract: Protein kinase C regulates the progressive muscle degeneration in Caenorhabditis elegans. In order to investigate the function of PKC involved in muscle degeneration, this paper cloned a cDNA isoform of pkc-2 gene of C.elegans and constructed the recombinant plasmids pPD118.20-pKG63 containing the isoform. The new isoform was then further studied for gene expression pattern. Immunocytochemistry experiment showed that this cDNA isoform expressed in body-wall muscle cells and located near the dense body.
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