Study on Bambuterol and Its Metabolite in Rat Serum by Capillary Electrophoresis with Amperometric Detection(Chinese)
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摘要: 以毛细管区带电泳安培检测法对大鼠血清中班布特罗及其活性代谢物特布他林的水平及药物代谢状况进行了研究.以直径300 μm的碳圆盘电极为工作电极，检测电位为1.10 V (vs. Ag/AgCl)，在0.02 mol·L-1磷酸盐缓冲体系中(pH 7.0)，当分离电压为10 kV时两种被分析物质在16 min内可基线分离.班布特罗和特布他林浓度分别在2.0×10-5~5.0×10-7mol·L-1和2.0×10-6~5.0×10-8mol·L-1范围内，与峰电流呈良好的线性关系，检测限分别可达2.5×10-7mol·L-1和2.0×10-8mol·L-1.将该法直接用于班布特罗及其活性代谢物特布他林在大鼠血清中的分离与测定，无需样品预处理，分析结果令人满意.该方法具有灵敏度高、简便、易操作等优点，在临床检验和药物动力学研究中具有很好的应用前景.Abstract: The amperometric detection of bambuterol and its metabolite in rat serum following their separation by capillary electrophoresis is described in this paper. A carbon disk electrode used as working electrode for two analytes exhibited a good response at 1.10 V (vs. Ag/AgCl). The optimal conditions of separation and detection were pH 7.0 phosphate buffer solution (0.02 mol·L-1 ), 10 kV for separation voltage. The peak current versus concentration was linear over the range of 2.0×10-5~5.0×10-7mol·L-1for bambuterol and 2.0×10-6~5.0×10-8mol·L-1for terbutaline. The detection limits for bambuterol and terbutaline were 2.5×10-7mol·L-1and 2.0×10-8mol·L-1respectively. This method was successfully applied to the determination of bambuterol and its active metabolite in rat serum.
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