Studies on the Antioxidation Effects of Alpha-Lipoic Acid and Dihydrolipoic Acid(Chinese)
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摘要: 采用多种化学发光体系和比色体系研究了氧化型硫辛酸——α-硫辛酸（α-lipoic acid, LA）和还原型硫辛酸——二氢硫辛酸（dihydrolipoic acid, DHLA）直接清除活性氧、抗脂质过氧化以及对#8226;OH引起的DNA氧化损伤的保护作用.实验发现，LA和DHLA能有效清除相应体系中的活性氧过氧化氢（H2O2）、羟自由基（#8226;OH）、过氧亚硝基阴离子（ONOO-）和二苯代苦味肼基自由基（DPPH#8226; ），能显著抑制脂质过氧化，并对#8226;OH引起的DNA氧化损伤有良好的保护作用，而DHLA还可以有效清除超氧阴离子自由基（O#8226;2-）.这些结果提示，LA和DHLA都是良好的抗氧化剂，但相比之下DHLA的抗氧化能力要显著强于LA.本研究为LA和DHLA保健和药用价值的进一步开发，提供了自由基生物学方面的依据.Abstract: Lipoic acid(LA) widely exists in all kinds of cells. It can be converted to dihydrolipoic acid (DHLA) in mammalian cell. The ability of alpha-LA and DHLA to scavenge reactive oxygen species and prevent DNA from oxidative damage by #8226;OH was evaluated using chemiluminescence systems and colorimetric systems. The experiments show that LA and DHLA can directly scavenge #8226;OH、H2O2、ONOO-and DPPH#8226; ，effectively inhibit lipid peroxidation and DNA oxidative damage by #8226;OH； DHLA can also effectively scavenge O#8226;2-. These results indicate that both LA and DHLA are effective antioxidants and the anti-oxidative ability of DHLA is obviously stronger than that of LA. The present investigation can provide new free radical biological data for the further study on the medical and health-care applications of LA and DHLA.
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