Screening of a phenanthrene-degrading bacterium and its microstructure of cell surface(Chinese)
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摘要: 从东北老工业基地长期受石油与重金属污染的土壤中，分离获得了一株以菲为唯一碳源的高效降解菌株ZX16，通过形态、生理生化和分子生物学鉴定为溶芳烃鞘氨醇单胞菌（Sphingomonas aromaticivorans）.菌株降解菲的特性研究结果表明，ZX16可耐受2 500 mg/L的菲，对1 500 mg/L的菲在72 h内降解率为98.2%.不同的重金属离子对菌株的降解效能影响大小依次为Cu2＋＞Cd2＋＞Zn2＋＞Pb2＋.菌株在中性条件下生长良好，原子力显微镜观察结果表明，菌株在复合污染物胁迫下细菌体积和形态有显著变化，细胞表面结构也从粗糙趋向光滑.Abstract: One strain which could use phenanthrene as sole carbon source was isolated from petroleum and heavy metal containminated soil,collected from old industry depot in northeast China.Based on morphological and physio-biochemical characteristics and homology identification of 16S rDNA sequence,the strain ZX16 was identified as Sphingomonas aromaticivorans.In the mineral salts medium under initial phenanthrene concentration of 1 500 mg/L the removal rates of phenanthrene was 98.2% within 72 h. Tolerance of ZX16 to phenanthrene exceeds 2 500 mg/L and ZX16 was relatively to the cell growth at neutral pH value. The effect of different heavy metal on degrading rate was Cu2＋＞Cd2＋＞Zn2＋＞Pb2＋in order. Microstructure of cell surface screening by Atomic Force Microscopy shows that the volume and morphous of strain have significant changes and the surface also changes from rough to smooth and glossy.
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