Polystyrene photonic crystal and a series of two-dimensional (2D) photonic crystals based on polystyrene template were prepared, including two-dimensional photonic crystal of α-Fe2O3 bowls and two-dimensional photonic crystal of ZnO pillars. Large-area specular reflectance measurements showed the presence of photonic stop band. Self-assembled PS is a simple, low cost and repeatability method to fabricate large-area two-dimensional photonic crystal. It offers a new idea on preparing two-dimensional photonic crystal.