Identification of wilt pathogens of potted Chongming narcissus(Narcissus tazetta var. chinensis)
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摘要: 依据柯赫氏法则对崇明水仙盆栽中叶褐枯病病原菌进行分离，得到3个可造成鳞茎和叶不同程度腐烂的致病真菌菌株.应用形态学鉴定法和真菌rDNAITS序列分析及ISSR分子标记技术进行鉴定，结果1株为丛赤壳属的丛赤壳菌（Nectria gliocladioides），2株为镰刀菌属的串珠镰刀菌胶胞变种（Fusarium moniliforme var. subglutinans）.其中 Nectria gliocladioides 为引起崇明水仙盆栽枯萎病的主要病原真菌，该菌的最适生长温度为25 ℃，低于5 ℃和高于40 ℃菌丝均不能生长.研究结果证实ISSR技术可用于区分菌株间的种属差异，可作为病原真菌鉴定的分子辅助手段.Abstract: Using morphologic characters and internal transcribed spacers (ITS) sequence analysis and Inter-simple-sequence -repeat（ISSR）markers, 3 isolates wilt pathogens in potted plant of Chongming narcissus were detected. One was Nectria gliocladioides , while other two were Fusarium moniliforme var. subglutinans . Pathogenic test resulted in that N.gliocladioides was the advantage pathogen, and its optimum growth temperature is 25 ℃. The hypha couldn’t growth while the temperature is lower than 5 ℃ or higher than 40 ℃. The study would be helpful to prevent and cure blight of Chongming narcissus potted plant. ISSR markers are useful for distinguishing the pathogen stains. It could be a molecular assistant means for identification of fungal and species.
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