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MA Tengfei, XU Zhen, FENG Linyan, LI Xiaofang, SUN Yue, XU Ling. Identification of wilt pathogens of potted Chongming narcissus(Narcissus tazetta var. chinensis)[J]. Journal of East China Normal University (Natural Sciences), 2010, (6): 64-71,8.
Citation:
MA Tengfei, XU Zhen, FENG Linyan, LI Xiaofang, SUN Yue, XU Ling. Identification of wilt pathogens of potted Chongming narcissus(Narcissus tazetta var. chinensis)[J]. Journal of East China Normal University (Natural Sciences), 2010, (6): 64-71,8.
MA Tengfei, XU Zhen, FENG Linyan, LI Xiaofang, SUN Yue, XU Ling. Identification of wilt pathogens of potted Chongming narcissus(Narcissus tazetta var. chinensis)[J]. Journal of East China Normal University (Natural Sciences), 2010, (6): 64-71,8.
Citation:
MA Tengfei, XU Zhen, FENG Linyan, LI Xiaofang, SUN Yue, XU Ling. Identification of wilt pathogens of potted Chongming narcissus(Narcissus tazetta var. chinensis)[J]. Journal of East China Normal University (Natural Sciences), 2010, (6): 64-71,8.
Using morphologic characters and internal transcribed spacers (ITS) sequence analysis and Inter-simple-sequence -repeat(ISSR)markers, 3 isolates wilt pathogens in potted plant of Chongming narcissus were detected. One was Nectria gliocladioides , while other two were Fusarium moniliforme var. subglutinans . Pathogenic test resulted in that N.gliocladioides was the advantage pathogen, and its optimum growth temperature is 25 ℃. The hypha couldn’t growth while the temperature is lower than 5 ℃ or higher than 40 ℃. The study would be helpful to prevent and cure blight of Chongming narcissus potted plant. ISSR markers are useful for distinguishing the pathogen stains. It could be a molecular assistant means for identification of fungal and species.