αCaMKII过量表达增强小鼠岛叶皮层兴奋性突触传递

刘汝清; 段燕红; 曹晓华

αCaMKII过量表达增强小鼠岛叶皮层兴奋性突触传递

1.
华东师范大学脑功能基因组学教育部及上海市重点实验室,上海 200062

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中图分类号: Q427
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出版历程
- 收稿日期: 2010-04-01
- 修回日期: 2010-07-01
- 刊出日期: 2011-07-05

αCaMKII overexpressions enhance excitatory synaptic transmission in insular cortex of mice

1.
Key Laboratories of MOE and STCSM Shanghai, Institute of Brain Functional Genomics，East China Normal University， Shanghai 200062， China

摘要

摘要: 为了研究CaMKII在小鼠前脑过量表达对岛叶皮层兴奋性锥体细胞基本电生理特性和突触传递的影响，利用脑片膜片钳技术研究CaMKII-F89G转基因小鼠岛叶皮层锥体细胞的基本电生理性质及突触传递.结果显示：野生型和CaMKII-F89G转基因型小鼠岛叶皮层锥体细胞在静息膜电位，动作电位及电流电压曲线方面没有显著性差异；在突触传递中，野生型鼠与转基因鼠的自发兴奋性突触后电流和微小兴奋性突触后电流的幅度差异显著，而频率没有显著性差异；此外，两组小鼠的双脉冲易化（PPF）曲线也没有显著性差异.由此得出结论，CaMKII在前脑特异性过量表达可能不改变岛叶皮层锥体细胞基本电生理特性和突触前递质释放能力；CaMKII过量表达提高自发兴奋性突触后电流幅度可能是通过突触AMPA 和（或）NMDA受体介导的.
- αCaMKII /
- 岛叶皮层 /
- 兴奋性突触后电流
Abstract: The experiment was designed to study basic electrophysiological characteristics and basal synaptic transmission of insular pyramidal cells in CaMKII forebrain overexpression mice by patch clamping. The basic electrophysiological results showed that there were no significant differences in the resting membrane potential, action potential and I-V curve of pyramidal cells in insular cortex between wild type and transgenic mice. No significant difference was measured in the frequency of sEPSCs and mEPSCs. However, compared to the wild type mice, transgenic mice exhibited augmented amplitude of sEPSCs and mEPSCs. There was also no significant difference in PPF curve. The results suggested that forebrain specific overexpression of CaMKII did not change the basic electrophysiological characteristics and presynaptic transmitter release in insular cortex. The influence of CaMKII overexpression on the amplitude of EPSCs may be mediated by postsynaptic receptors.
- αCaMKII /
- Insular cortex /
- EPSCs

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