Influence of nitrogen and phosphorus concentrations on the growth characteristics of Microcystis aeruginosa
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摘要: 在不同氮(N)、磷(P)初始浓度的培养液中对铜绿微囊藻(Microcystis aeruginosa)进行培养.利用Monod方程分别计算铜绿微囊藻对氮、磷的半饱和常数(Ks).结果表明:以单一元素为限制底物时,满足铜绿微囊藻正常生长的氮浓度大于4.0 mgL-1,磷浓度大于0.50 mgL-1;铜绿微囊藻最适生长的氮浓度范围为32.0~64.0 mgL-1,磷浓度范围为1.0~1.50 mgL-1;以磷为限制底物时的半饱和常数Ksp远远小于以氮为限制底物时的半饱和常数KsN(KsNKsp),说明铜绿微囊藻对磷的亲和性高于氮.与铜绿微囊藻最大现存量(X)呈高度线性相关时的氮浓度范围为0.20~64.0 mgL-1,磷浓度范围为0.02~1.50 mgL-1.铜绿微囊藻比增长速率()连续增加的氮浓度范围为0.20~1.60 mgL-1,磷浓度范围为0.02~0.50 mgL-1.Abstract: Microystis aeruginosa was cultured in solutions of different initial nitrogen and phosphorus concentrations. The semi-saturation constants (Ks) of Microystis aeruginosa to P and N were calculated respectively by using the Monod equation. The results show that the Microystis aeruginosa grew normally when the concentrations of N and P were greater than 4.0 mgL-1 and mgL-1 respectively. The optimum concentration ranges of N and P for Microystis aeruginosa growth were 32.0~64.0 mgL-1 and 1.0~1.50 mgL-1 respectively. KsN is higher than Ksp, which indicates that the affinity of P to Microystis aeruginosa is higher than N. When the concentration ranges of N and P were 0.20~64.0 mgL-1 and 0.02~1.50 mgL-1 respectively,they had high liner correlation with the largest extent quantity of Microystis aeruginosa. The special growth rate increases continuously when the concentration ranges of N and P are 0.20~1.60 mgL-1 and 0.02~0.50 mgL-1 respectively.
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Key words:
- Microcystis aeruginosa /
- nitrogen /
- phosphorus /
- growth characteristics
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[1] [1]ALEKSANDRA M O. Effect of eutrophication on changes in the composition of zooplankton in the Grosnica Reservoir (Serbia, Yugoslavia) [J].Hydrobiologia, 2000, 436: 171-178.[2]MAGADZA C H D. Evaluation of eutrophication control in Lake Chivero, Zimbabwe, by multivariate analysis of zooplankton [J]. Hydrobiologia, 1994, 272: 277-292.[3]GREGOR K S V. Eutrophication and endangered aquatic plants:an experimental study on Baldellia ranunculoides (L.) Parl.(Alismataceae) [J]. Hydrobiologia, 2009, 635: 181-187.[4]金相灿.中国湖泊环境[M].北京:海洋出版社, 1995.[5]LU Y, WEN J F. Isolation pure cultivation and total DNA extraction of Microcystis aeruginosa Kütz in Dianchi Lake[J]. Journal of Sciences, 2001, 13(3): 285-288.[6]刘丽萍.滇池水华特征及成因分析[J]. 环境科学研究,1999,12(5):36-37.[7]陈宇炜,高锡云,陈伟民,等.〖JP2〗太湖微囊藻的生长特征及其分离纯培养的初步研究[J]. 湖泊科学,1999,11(4):351-356.[8]张红,黄勇.基于NOAA/AVHRR卫星资料的巢湖水华规律分析中国环境科学[J]. 湖泊科学,2009,29(7):727-732.[9]阮仁良,王云.淀山湖水环境质量评价及污染防治研究[J].湖泊科学, 1993, 5(2):153-158.[10] FRANCIS G. Poisonous Australian Lake[J]. Nature, 1978, 18: 11-12.[11] SCHWIMMER D, SCHWIMMER M. Algae and Man[M]. New York: Plenum Press, 1964: 368-412.[12] CHWIMMER M, SCHWIMMERV D. Algae, Man and Environment[M]. New York: Syracuss Univ Press, 1968: 278-358.[13] GORHAM P R, CARMICHAEL W W. Phycotoxins from blue-green algae[J]. Pure Appl Chem, 1979, 52: 165-174.[14] 郑朔方,杨苏文,金相灿.铜绿微囊藻生长的营养动力学[J].环境科学, 2005, 26(2): 152-156.[15] 陈明曦.蓝藻水华生消机制室内模拟试验研究[D]. 湖北宜昌:三峡大学, 2007.[16] 李夜光,李中奎,耿亚红,等. 富营养化水体中N、P浓度对浮游植物生长繁殖速率和生物量的影响[J]. 生态学报. 2006, 26(2):317-32.[17] 金相灿,屠清瑛.湖泊富营养化调查规范[M].2版.北京:中国环境科学出版社, 1990.[18] 张自杰,周帆.活性污泥反应动力学[M].北京:中国环境科学出版社, 1989.[19] 郑忠明,白培峰,陆开宏,等.铜绿微囊藻和四尾栅藻在不同温度下的生长特性及竞争参数计算[J].水生生物学报, 2008,32(5):720-728.[20] 程曦,李小平.淀山湖氮磷营养物长期变化规律及其对藻类增长影响研究[J].上海环境科学, 2008,27(1):9-16.[21] 彭近新.水质富营养化与防治[M].北京:中国环境科学出版社, 1988.[22] 许海,杨林章,茅华,等.铜绿微囊藻、斜生栅藻生长的磷营养动力学特征[J]. 生态环境, 2006, 15(5):921-924.[23] 许海,杨林章,刘兆普.铜绿微囊藻和斜生栅藻生长的氮营养动力学特征[J]. 环境科学研究, 2008, 21(1):69-73.
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