Expressions of REGγ, p53, NOTCH1 and BRAF gene in thyroid cancer and their clinical relevance
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摘要: 旨在探讨〖WTBX〗REG,p〖STBX〗53,NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗在乳头状甲状腺癌中的表达及其与临床病理因素的相关性.应用Realtime-PCR及免疫组织化学方法检测〖WTBX〗REG,p〖STBX〗53,NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗在乳头状甲状腺癌组织中的表达,分析〖WTBX〗REG〖WTBZ〗与〖WTBX〗〖STBX〗p53,NOTCH1〖STBZ〗,BRAF〖WTBZ〗之间的关系及其与临床病理因素的相关性.〖WTBX〗REG〖WTBZ〗在乳头状甲状腺癌组织中表达明显增高,且其阳性率为87.5%(35/40).〖WTBX〗REG〖WTBZ〗表达与患者年龄、淋巴结转移情况有关(〖WTBX〗P〖WTBZ〗=0.015 10.05),与患者性别无显著性相关(〖WTBX〗P〖WTBZ〗=0.7420.05);〖WTBX〗REG〖WTBZ〗表达与〖WTBX〗〖STBX〗p53,NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗表达呈正相关(〖WTBX〗P〖WTBZ〗=0.023 30.05).〖WTBX〗REG〖WTBZ〗阳性表达可作为乳头状甲状腺癌发生、发展的生物学指标,联合〖WTBX〗〖STBX〗p53〖WTBZ〗、〖WTBX〗〖STBX〗NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗检测可以为临床诊治及预后判断提供参考.Abstract: This study was designed to investigate the expressions of〖WTBX〗 REG〖WTBZ〗,〖WTBX〗 〖STBX〗p53〖WTBZ〗, 〖WTBX〗NOTCH1〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 in papillary thyroid cancer as well as the potential correlations with clinicopathological significance. The expressions of 〖WTBX〗REG〖WTBZ〗,〖WTBX〗 p53〖WTBZ〗,〖WTBX〗 NOTCH1〖STBZ〗〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 in thyroid cancer samples were detected by Realtime-PCR and immunohistochemistry, respectively. The correlations with clinicopathological factors were statistically analyzed. The expression of 〖WTBX〗REG〖WTBZ〗 in papillary thyroid cancer samples was significantly higher, with an 87.5% (35/40) positive rate in cancer tissues, and was correlated with age and lymph node status (〖WTBX〗P〖WTBZ〗=0.015 10.05), but not related to sex (〖WTBX〗P〖WTBZ〗=0.7420.05). In addition, the 〖WTBX〗REG〖WTBZ〗 had a positive correlation with the levels of 〖WTBX〗〖STBX〗p53, NOTCH1〖STBZ〗〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 (〖WTBX〗P〖WTBZ〗=0.023 30.05), indicating that 〖WTBX〗REG〖WTBZ〗 could serve as an objective biological-marker for estimating the occurrence and progression of papillary thyroid cancer. Combined detection of 〖WTBX〗〖STBX〗p53〖WTBZ〗,〖WTBX〗 NOTCH1〖WTBZ〗 and 〖WTBX〗BRAF 〖WTBZ〗may facilitate clinical diagnosis, treatment and prediction of prognosis.
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Key words:
- thyroid cancer /
- p53 /
- NOTCH1 /
- REG /
- BRAF
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[1] [1] OKAMURA T,TANIGUCHI S,OHKURA T,et al.Abnormally high expression of proteasome activator-gamma in thyroid neoplasm[J]. J Clin Endocrinol Metab, 2003, 88(3): 1374-1383.[2] RODERICK M,HELEN G,PAOLO G, et al. Evidence that one subset of anaplastic thyroid carcinomas are derived from papillary carcinomas due to BRAF and 〖STBX〗p53〖STBZ〗 mutations[J]. CANCER, 2005, 103(11): 2261-2268.[3] KIMURA E T, NIKIFOROVA M N, ZHU Z, et al. High prevalence of 〖WTBX〗BRAF〖WTBZ〗 mutations in thyroid cancer genetic evidence for constitutive activation of the RET/PTC-RAS-BRAF signaling pathway in papillary thyroid carcinoma[J]. CANCER RESEARCH, 2003, 63: 1454-1457.[4] PRATHIBHA R, WEAVER K L, CAPOBIANCO A J. Notch signalling in solid tumours: a little bit of everything but not all the time[J]. Nature, 2011(11): 338-351.[5] NIKIFOROVA M N, KIMURA E T, GANDHI M, et al. BRAF mutations in thyroid tumors are restricted to papillary carcinomas and anaplastic or poorly differentiated carcinomas arising from papillary carcinomas[J]. J Clin Endocrinol Metab, 2003, 88(11): 5399-5540.[6] 沈铭昌.免疫组化在病理学中的应用进展与评价[J].实用肿瘤杂志, 2002, 17(5): 293-294.[7] MAO I, LIU J, LI X, et al. REGgamma, a proteasome activator and beyond[J]. Cell Mol Life Sci, 2008, 65(24): 3971-80.[8] NIKAIDO T, SHIMADA K, SHIBATA M, et al. Cloning and nucleotide sequence of cDNA for Ki antigen, a highly conserved nuclear protein detected with sera from patients with systemic lupus erythematosus[J]. Clin Exp Immunol, 1990, 79(2): 209-214.[9] ROESSLER M, ROLLINGER W, MANTOVANI-ENDL L,et,al. Identification of PSME3 as a novel serum tumor marker for colorectal cancer by combining two-dimensional polyacrylamide gel electrophoresis with a strictly mass spectrometry-based approach for data analysis[J]. Mol Cell Proteomics, 2006, 5(11): 2092-2101.[10] OKAMURA T, TANIGUCHI S, OHKURA T,et al. Abnormally high expression of proteasome activator-gamma in thyroid neoplasm[J]. J Clin Endocrinol Metab, 2003, 88(3): 1374-1383.[11] SALVATORE G, NAPPI TC, SALERNO P, et al. A cell proliferation and chromosomal instability signature in anaplastic thyroid carcinoma[J]. Cancer Res, 2007, 67(21): 10148-10158.[12] DAVIDOFFf A M, HEMDON J E, GLOVER N S, et al. Relation between 〖STBX〗p53〖STBZ〗 overexpression and established prognostic factors in breast cancer[J]. Surgery, 1991, 110(2): 259-264.[13] ZHANG Z, ZHANG R.Proteasome activator PA28 gamma regulates 〖STBX〗p53〖STBZ〗 by enhancing its MDM2-mediated degradation[J]. EMBO J, 2008, 27(6): 852-64.[14] PEYSSONNAUX C,EYCHENE A. The RAF/MEK/ERKpathway: new concepts of activation[J]. Biol Cell, 2001, 93: 53-62.[15] DUESBERY N S,WEBB C P,VANDE W G F.MEK wars,a new front in the battle against cancer[J]. Nat Med, 1999(5): 736-742.[16] PARR C,WATKINS G, JIANG W G. The possible correlation of Notch-1 and Notch-2 with clinical outcome and tumer clinicopathological parameters in human breast cancer[J]. Int J Med, 2004, 14(5): 779-786.[17] QI R, AN H, YU Y, et al. 〖STBX〗NOTCH1〖STBZ〗 signaling inhibits growth of human hepatocellular crcinoma through induction of cell cycle arrest and apoptosis[J]. Cancer Res, 2003, 63: 8323-8329.
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