Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility
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摘要: 测定了原花青素(PC)交联的脱细胞牛心包膜(dbpECM)的力学性能、稳定性和血液相容性.实验结果显示,2.5 mg/mL原花青素交联能将拉伸强度从29.1 MPa提高到51.3 MPa,并保持弹性模量基本不变,而抗胶原酶降解能力与戊二醛相当;PC-dbpECM浸泡于D-Hanks溶液中,仅过量未交联原花青素在一周内释放,而交联的PC在PC-dbpECM中保持稳定.1 mg/mL PC即可基本抑制体外钙化.PC或GA交联的dbpECM都有较好的血液相容性,但PC交联明显优于GA. 2.5 mg/mL PC交联的dbpECM血小板的粘附率(7.8%)显著低于未交联组(26.1%)和GA交联组(36.6%),表明PC-dbpECM具有较好的抗血栓形成能力.结果表明PC-dbpECM具有良好的力学性能,稳定性和血液相容性,有可能成为制备人工心脏瓣膜的优良材料.Abstract: In this study, decellularized bovine pericardium matrix (dbpECM) was cross-linked by using PC at series concentrations. Mechanical properties, stability and hemo-compatibility of PC-crosslinked dbpECM (PC-dbpECM) were investigated. The results show that the tensile strength of the PC-dbpECM which crosslinked by the 2.5 mg/mL PC is increased from 29.1 MPa to 51.3 MPa with the elastic modulus roughly at basic level. Resistance of PC-dbpECM to collagenase hydrolysis is equivalent to that of GA-crosslinked. When PC-dbpECM soaked in D-Hanks solution, only the excessive non-crosslinked PC released within a week, leaving the crosslinked PC stable all the experiment period. PC crosslinked dbpECM (1 mg/mL) can effectively inhibit calcification in vitro. Both PC- and GA-dbpECM have ideal hemo-compatibility, but the PC cross-linking is superior to that of GA. The rate of platelet adhesion on the surface of 2.5 mg/mL PC crosslinked dbpECM (7.8%) was significantly lower than that of non-crosslinked group (26.1%) and the GA crosslinked group (36.6%), indicating that PC-dbpECM has low coagulation spotential. These data suggest that PC-dbpECM has good mechanical properties, stability and hemo-compatibility, suitable for preparing biological heart valves.
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