Analysis of sugar metabolism and its related enzymes activities during vase-holding days of cut rose petal
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摘要: 为探究糖代谢及其酶对月季脱离母体后的生命活动的影响,以月季(Rosa hybrida Hort)切花黑魔术(cv. Black Magic flower)和影星(cv. Movie Star flower)为试验材料,分析瓶插期间花瓣糖代谢及其相关酶活性的变化.试验表明:随着月季切花瓶插时间的延长和花瓣开放,花瓣中葡萄糖(G)和果糖(F)含量显著增加,蔗糖含量随之降低,切花影星的己糖(G+F)含量、花径的增长和花朵鲜重都明显高于黑魔术;花瓣中酸性转化酶(AI)活性与中性转化酶(NI)、蔗糖合成酶(SS)和磷酸蔗糖合成酶(SPS)相比具有较高的水平,AI活性与蔗糖降解指数(SDI)成正相关,AI是降解蔗糖并积累己糖的关键酶,而花瓣中己糖积累在月季切花生长发育过程中起重要作用.Abstract: Analyze changes of sugar metabolism and its related enzymes activities during vase-holding days of cut roses named cv. Black Magic flower and cv. Movie Star flower were studied. The results showed that glucose and fructose contents increased rapidly with the cell enlargement in the petals and during flower growth, but the content of sucrose dropped gradually. The cut rose named cv. Movie Star flower had a larger hexoses (glucose+fructose) content , fresh weight and a bigger flower diameter fresh weight than cv. Black Magic flower . The activity of acid invertase (AI) was always at a higher level than those of the neutral invertase (NI), sucrose synthase (SS) and sucrose phosphate synthase (SPS). The activity of acid invertase (AI) was positively correlated with sucrose degradation index (SDI). In conclusion, the acid invertase (AI) was a key enzyme in the sucrose degradation and hexoses accumulation, while hexose accumulation in the petal might be important for flower growth and development in cut rose.
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Key words:
- cut roses petals /
- sugar metabolism /
- enzymes activities
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