过量表达URO基因抑制拟南芥次生细胞壁开关基因表达

倪伟平; 庞静洋; 徐艳飞; 李玲; 李小方; 孙越

过量表达URO基因抑制拟南芥次生细胞壁开关基因表达

1.
华东师范大学生命科学学院, 上海 200241

详细信息

中图分类号: Q945
计量
- 文章访问数: 1517
- HTML全文浏览量: 18
- PDF下载量: 1540
- 被引次数: 0
出版历程
- 收稿日期: 2013-03-01
- 修回日期: 2013-06-01
- 刊出日期: 2014-05-25

Overexpression of URO gene repress the master switches of secondary cell wall development in Arabidopsis

1.
School of Life Sciences, East China Normal University, Shanghai 200241, China

摘要

摘要: 植物次生细胞壁的发育受到一系列转录调控因子的调节.但关于这一过程如何与植物整体发育相整合，一直少有报道.拟南芥URO (UP-RIGHT ROSETTE) 基因过量表达导致在相应突变体uro中自由态生长素浓度明显升高，并导致该突变体中次生细胞壁发育减弱.本研究利用突变体uro及其杂合子uro/+，通过定量PCR的方法，检测了一系列调控植物次生细胞壁分化相关基因的表达情况.结果表明，在uro/uro和uro/+中，植物次生细胞壁发育开关基因的表达受到明显抑制，而对于木质部导管细胞分化的开关基因没有明显影响.因此，URO基因是介导植物细胞壁次生加厚与植物生长发育状态关联的基因，URO基因通过抑制次生细胞壁发育达到维持细胞的生长状态的效果.
- 次生细胞壁 /
- 生长素 /
- 开关基因 /
- URO(UP-RIGHT ROSETTE)基因
Abstract: There are several transcriptional factors that have been found taking part in secondary cell wall differentiation as master switches in Arabidopsis. However, how these master switches link to the major development event of plant is still unknown. Overexpression of URO (UP-RIGHT ROSETTE) gene leads to much higher free IAA concentration in uro mutant than that in wild type. In this work, the expression levels of the master switch genes were checked in uro/uro and uro/+ mutants through quantity PCR reactions. The results show that expressions of the master switch genes were significantly repressed while the expression of xylem development master switcher genes were not effected when URO gene was over expressing in uro/uro and uro/+ mutants. It can be concluded that URO gene takes part in the integration of secondary cell wall differentiation with the major development process of plant and URO gene could keep plant cell in a growth manner through inhibition of the secondary cell wall development.
- secondary cell wall /
- auxin /
- master switches /
- URO(UP-RIGHT ROSETTE) gene

HTML全文

参考文献(1)

[1]

［1］ WANG H Z, DIXON R A. On-off switches for secondary cell wall biosynthesis［J］. Molecular Plant, 2012, 5(2): 297-303.

［2］ ZHONG R, LEE C, YE Z H. Evolutionary conservation of the transcriptional network regulating secondary cell wall biosynthesis［J］. Trends in Plant Science, 2010, 15(11): 625-632.

［3］ MITSUDA N, SEKI M, SHINOZAKI K, et al.The NAC transcription factors 〖STBX〗NST1 and NST2〖STBZ〗 of Arabidopsis regulate secondary wall thickenings and are required for anther dehiscence［J］. Plant Cell, 2005, 17(11): 2993-3006.

［4］ MITSUDA N, IWASE A, YAMAMOTO H, et al. NAC transcription factors, 〖STBX〗NST1 and NST3,〖STBZ〗 are key regulators of the formation of secondary walls in woody tissues of Arabidopsis［J］. Plant Cell, 2007, 19(1): 270-280.

［5］ ZHONG R, DEMURA T, YE Z H. 〖STBX〗SND1,〖STBZ〗 a NAC domain transcription factor, is a key regulator of secondary wall synthesis in fibers of Arabidopsis［J］. Plant Cell, 2006, 18(11): 3158-3170.

［6］ OHASHI-ITO K, ODA Y, FUKUDA H. Arabidopsis VASCULAR-RELATED 〖STBX〗NAC-DOMAIN6〖STBZ〗 directly regulates the genes that govern programmed cell death and secondary wall formation during xylem differentiation［J］. Plant Cell, 2010, 22(10): 3461-3473.

［7］ YAMAGUCHI M, MITSUDA N, OHTANI M, et al. VASCULAR-RELATED NAC-DOMAIN 〖STBX〗7〖STBZ〗 directly regulates the expression of a broad range of genes for xylem vessel formation［J］. Plant Journal, 2011, 66(4): 579-590.

［8］ KUBC M, UDAGAWA M, NISHIKUBO N, et al. Transcription switches for protoxylem and metaxylem vessel formation［J］. Genes & Development, 2005, 19(16):1855-1860.

［9］ ZHONG R, LEE C, YE Z H. Global analysis of direct targets of secondary wall NAC master switches in Arabidopsis［J］. Molecular Plant, 2010, 3(6): 1087-1103.

［10］ ZHONG R, YE Z H. 〖STBX〗MYB46 and MYB83〖STBZ〗 bind to the SMRE sites and directly activate a suite of transcription factors and secondary wall biosynthetic genes［J］. Plant and Cell Physiology, 2012, 53(2): 368-380.

［11］ ZHONG R,LEE C, ZHOU J, et al. A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis［J］. Plant Cell, 2008, 20(10): 2763-2782.

［12］ MCCARTHY R L, ZHONG R, YE Z H. 〖STBX〗MYB83〖STBZ〗 is a direct target of 〖STBX〗SND1〖STBZ〗 and acts redundantly with 〖STBX〗MYB46〖STBZ〗 in the regulation of secondary cell wall biosynthesis in Arabidopsis［J］. Plant and Cell Physiology, 2009, 50(11): 1950-1964.

［13］ ZHAO Q, WANG H, YIN Y, et al. Syringyl lignin biosynthesis is directly regulated by a secondary cell wall master switch［J］. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(32): 14496-14501.

［14］ ZHOU J, LEE C, ZHONG R, et al. 〖STBX〗MYB58 and MYB63〖STBZ〗 are transcriptional activators of the lignin biosynthetic pathway during secondary cell wall formation in Arabidopsis［J］. Plant Cell, 2009, 21(1): 248-266.

［15］ ZHAO Q, DIXON R A. Transcriptional networks for lignin biosynthesis: more complex than we thought［J］. Trends in Plant Science, 2011, 16(4): 227-233.

［16］ SULLIVAN S, RALET M C, BERGER A, et al. 〖STBX〗SA5〖STBZ〗 is required for the synthesis of cellulose with a role in structuring the adherent mucilage of Arabidopsis seeds［J］. Plant Physiology, 2011, 156(4): 1725-1739.

［17］ YORK W S, O′NEILL M A. Biochemical control of xylan biosynthesis-which end is up［J］. Current Opinion in Plant Biology, 2008, 11(3): 258-265.

［18］ BERTHET S, DEMONT-CAULET N, POLLET B, et al. Disruption of 〖STBX〗LACCASE4〖STBZ〗 and 17 results in tissue-specific alterations to lignification of Arabidopsis thaliana stems［J］. Plant Cell, 2011, 23(3): 1124-1137.

［19］ GUO Y L, YUAN Z, SUN Y, et al. Characterizations of the uro mutant suggest that the URO gene is involved in the auxin action in Arabidopsis［J］. Acta Botanica Sinica, 2004, 46(7): 846-853.

［20］ YUAN Z, YAO X, ZHANG D, et al. Genome-wide expression profiling in seedlings of the Arabidopsis mutanturo that is defective in the secondary cell wall formation［J］. Journal of Integrative Plant Biology, 2007, 49(12): 1754-1762.

［21］ SUN Y, YANG Y, YUAN Z, et al. Overexpression of the Arabidopsis gene UPRIGHT ROSETTE reveals a homeostatic control for indole-3-acetic acid［J］. Plant Physiology, 2010, 153(3): 1311-1320.

［22］ HUANG H, TUDOR M, WEISS C A, et al. The Arabidopsis MADS-box gene AGL3 is widely expressed and encodes a sequence-specific DNA-binding protein［J］. Plant Molecular Biology, 1995, 28(3): 549-567.

［23］ ZHONG R, RICHARDSON E A, YE Z H. Two NAC domain transcription factors, 〖STBX〗SND1 and NST1,〖STBZ〗 function redundantly in regulation of secondary wall synthesis in fibers of Arabidopsis［J］. Planta, 2007, 225(6): 1603-1611.

［24］ WANG J, ELLIOTT J E, WILLIAMSON R E. Features of the primary wall CESA complex in wild type and cellulose-deficient mutants of Arabidopsis thaliana［J］. Journal of Experimental Botany, 2008, 59(10): 2627-2637.

［25］ LEE C, O′NEILL M A, TSUMURAYA Y, et al. The irregular 〖STBX〗xylem9〖STBZ〗 mutant is deficient in xylan xylosyltransferase activity［J］. Plant and Cell Physiology, 2007, 48(11): 1624-1634.

［26］ PENA M J, ZHONG R, ZHOU G K, et al. Arabidopsis irregular 〖STBX〗xylem8 and irregular xylem9:〖STBZ〗 Implications for the complexity of glucuronoxylan biosynthesis［J］. Plant Cell, 2007, 19(2): 549-563.

［27］ BROWN D M, GOUBET F, VICKY W W A, et al. Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis［J］. Plant Journal, 2007, 52(6): 1154-1168.

［28］ ZHONG R Q, PENA M J, ZHOU G K, et al. Arabidopsis fragile 〖STBX〗fiber8,〖STBZ〗 which encodes a putative glucuronyl transferase, is essential for normal secondary wall synthesis［J］. Plant Cell, 2005, 17(12): 3390-3408.

［29］ BESSEAU S, HOFFMANN L, GEOFFROY P, et al. Flavonoid accumulation in arabidopsis repressed in lignin synthesis affects auxin transport and plant growth［J］. The Plant Cell Online, 2007, 19(1): 148-162.

［30］ LI X, BONAWITZ N D, WENG J K, et al. The growth reduction associated with repressed lignin biosynthesis in Arabidopsis thaliana is independent of flavonoids［J］. The Plant Cell Online, 2010, 22(5): 1620-1632.

［31］ YAN C, SHEN H, LI Q, et al. A novel ABA-hypersensitive mutant in Arabidopsis defines a genetic locus that confers tolerance to xerothermic stress［J］. Planta, 2006, 224(4): 889-899.

［32］ INOUE K, SEWALT V J H, MURRAY BALLANCE G, et al. Developmental expression and substrate specificities of alfalfa caffeic acid 3-O-Methyltransferase and caffeoyl Coenzyme a 3-O-Methyltransferase in relation to lignification［J］. Plant Physiology, 1998, 117(3): 761-770.

［33］ FELLENBERG C, OHLEN M, HANDRICK V, et al. The role of 〖STBX〗CCoAOMT1 and COMT1〖STBZ〗 in Arabidopsis anthers［J］. Planta, 2012, 236(1): 51-61.

［34］ VANHOLME R, STORME V, VANHOLME B, et al. A systems biology view of responses to lignin biosynthesis perturbations in Arabidopsis［J］. The Plant Cell Online, 2012, 24(9): 3506-3529.

［35］ DO C T, POLLET B, THéVENIN J, et al. Both caffeoyl Coenzyme A 3-O-methyltransferase 1 and caffeic acid O-methyltransferase 1 are involved in redundant functions for lignin, flavonoids and sinapoyl malate biosynthesis in Arabidopsis［J］. Planta, 2007, 226(5): 1117-1129.

［36］ LEV-YADUN S, WYATT S E, FLAISHMAN M A, The inflorescence stem fibers of Arabidopsis thaliana revoluta 〖STBX〗(ifl1)〖STBZ〗 mutant［J］. Journal of Plant Growth Regulation, 2004, 23(4): 301-306.

［37］ ZHONG R Q, YE Z H. Amphivasal vascular bundle 1, a gain-of-function mutation of the 〖STBX〗IFL1/REV〖STBZ〗 gene, is associated with alterations in the polarity of leaves, stems and carpels［J］. Plant and Cell Physiology, 2004, 45(4): 369-385.

［38］ ZHONG R Q, YE Z H. Alteration of auxin polar transport in the Arabidopsis 〖STBX〗ifl1〖STBZ〗 mutants［J］. Plant Physiology, 2001, 126(2): 549-563.

［39］ RATCLIFFE O J, RIECHMANN J L, ZHANG J Z. INTERFASCICULAR FIBERLESS1 is the same gene as REVOLUTA［J］. Plant Cell, 2000, 12(3): 315-317.

［40］ ZHONG R Q, YE Z H. 〖STBX〗IFL1,〖STBZ〗 a gene regulating interfascicular fiber differentiation in Arabidopsis, encodes a homeodomain-leucine zipper protein［J］. Plant Cell, 1999, 11(11): 2139-2152.

［41］ FUNK V, KOSITSUP B, ZHAO C S, et al. The Arabidopsis xylem peptidase 〖STBX〗XCP1〖STBZ〗 is a tracheary element vacuolar protein that may be a papain ortholog［J］. Plant Physiology, 2002,128(1): 84-94.

［42］ AVCI U, PETZOLD H E, ISMAIL I O, et al. Cysteine proteases 〖STBX〗XCP1 and XCP2〖STBZ〗 aid micro-autolysis within the intactcentral vacuole during xylogenesis in Arabidopsis roots［J］. Plant Journal, 2008, 56(2): 303-315.

［43］ PEREZ-AMADOR M A, ABLER M L, DE ROCHER E J, et al. Identification of 〖STBX〗BFN1,〖STBZ〗 a biofunctional nuclease induced during leaf and stem senescence in Arabidopsis［J］. Plant Physiology, 2000, 122(1): 169-179.

［44］ MURRAY J A H, JONES A, GODIN C, et al. Systems analysis of shoot apical meristem growth and development: integrating hormonal and mechanical signaling［J］. The Plant Cell Online, 2012, 24(10): 3907-3919.

［45］ SáNCHEZ-RODRíGUEZ C, RUBIO-SOMOZA I, SIBOUT R, et al. Phytohormones and the cell wall in Arabidopsis during seedling growth［J］. Trends in Plant Science, 2010, 15(5): 291-301.

［46］ ZHONG R, YE Z H. IFL1, a Gene regulating interfascicular fiber differentiation in Arabidopsis, encodes a homeodomain-leucine zipper protein［J］. The Plant Cell, 1999, 11(11): 2139-2152.

［47］ BERLETH T, MATTSSON J, HARDTKE C S. Vascular continuity and auxin signals［J］. Trends in Plant Science, 2000, 5(9): 387-393.

［48］ ZHANG M, ZHENG X, SONG S, et al. Spatiotemporal manipulation of auxin biosynthesis in cotton ovule epidermal cells enhances fiber yield and quality［J］. Nat Biotech, 2011, 29(5): 453-458.

施引文献

资源附件(0)

访问统计