PFOS暴露对秀丽线虫寿长的影响及其与IIS相关基因的关联

李丹; 武婧轩; 李嘉; 何德富

doi:10.3969/j.issn.1000-5641.2015.02.013

PFOS暴露对秀丽线虫寿长的影响及其与IIS相关基因的关联

doi: 10.3969/j.issn.1000-5641.2015.02.013

李丹^1,2,
武婧轩^1,2,
李嘉^1,2,
何德富^1,2, ,,

1.
华东师范大学生态与环境科学学院,上海200241;
2.
华东师范大学上海市城市化生态过程与生态恢复重点实验室,上海200241

基金项目:

国家科技重大专项（2013ZX0731000104）;上海市自然科学基金（11ZR1410500）;河口海岸国家重点实验室开放课题（SKLECKF201311）

详细信息

作者简介:
李丹，女，硕士研究生，研究方向为环境污染与健康.

通讯作者:
何德富，男，博士，副教授，研究方向为毒理学、环境与健康等.

中图分类号: X592
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- 被引次数: 0
出版历程
- 收稿日期: 2014-09-12
- 刊出日期: 2015-03-25

Effects of PFOS exposure on lifespan in C. elegans and their relations with IIS genes

LI Dan^1,2,
WU Jing-xuan^1,2,
LI Jia^1,2,
HE De-fu^{1,2
, ,},


1.
School of Ecological and Environmental Sciences, East China Normal University, Shanghai200241, China;
2.
Shanghai Key Lab for Urban Ecological Processes and Eco Restoration, East China Normal University, Shanghai200241, China

摘要

摘要: 以秀丽隐杆线虫（C.elegans）为模型，本文研究了全氟辛烷磺酸（PFOS）对机体寿长的影响及初步机理.结果显示0.2~200 mol/L的PFOS暴露50 h导致野生型秀丽线虫寿长呈剂量依赖性缩短.在4类转基因线虫上，观察到Insulin/IGF-l.1信号通路（IIS）相关的daf-16、daf-2和age-1基因突变或敲除能影响线虫的寿长.进一步观察PFOS暴露导致4类转基因线虫的寿长变化率，并与野生型线虫比较.在CF1139和CF1580突变种上daf16或daf2的突变均未改变PFOS的缩短寿长效应.而在CF1295和TJ1052转基因型上发现daf-16b的基因敲除或age-1基因突变阻断PFOS的减寿效应.结果表明PFOS慢性暴露能加速动物衰老，缩短寿长.PFOS作用与IIS信号通路关系密切，daf-16b和age-1基因在其中起重要作用.
- PFOS /
- 秀丽隐杆线虫 /
- 寿长 /
- Insulin/IGF-l.1信号通路 /
- daf-16b /
- age-1
Abstract: Using a model of C.elegans, this study aims to investigate the effect of PFOS on the lifespan and its mechanisms. Results showed that 0.2~200 mol/L PFOS shortened the lifespan of wild type wormsin a concentrationdependent manner after 50 h exposure.In four transgenic strains, mutation of daf-16, daf-2 and age-1 which are related to Insulin/IGFl.1pathway(IIS), could affect the lifespans of nematodes. We further observed lifespan change rates of the four transgene C.elegansafter exposed to PFOS.Results showed that mutation of daf-16 or daf-2 did not alter PFOS induced effects of shortening lifespan in CF1139 and CF1580 strain.However, knockout of daf-16b or mutation of age-1blocked PFOS induced effects of shortening lifespan in CF1295 and TJ1052 strain. Our results suggested that PFOS could accelerate aging and shorten the lifespan of nematodes. PFOSinduced effects are closely relative with IIS signaling pathways, in which daf-16b and age-1 play important roles.
- PFOSC.elegans lifespan IIS signaling pathway daf-16b age-1 /

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参考文献(1)

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