异养硝化-好氧反硝化细菌的筛选及其脱氮性能研究

张婷月; 丁钰; 黄民生

doi:10.3969/j.issn.1000-5641.2018.06.003

异养硝化-好氧反硝化细菌的筛选及其脱氮性能研究

doi: 10.3969/j.issn.1000-5641.2018.06.003

张婷月^1,2,,
丁钰^1,2,
黄民生^1,2, ,

1.
华东师范大学生态与环境科学学院, 上海 200241
2.
华东师范大学上海市城市化生态过程与生态恢复重点实验室, 上海 200241

基金项目:

国家重大科技专项 2014ZX07101102

国家重大科技专项 2017ZX07207001

上海市普陀区高层次人才科研创新项目 2014-A-18

地理信息科学教育部重点实验室开放研究基金 KLGIS2016A03

详细信息

作者简介:
张婷月, 女, 硕士研究生, 研究方向为水环境治理与修复.E-mail:1391994089@qq.com

通讯作者:
黄民生, 男, 教授, 博士生导师, 研究方向为水环境治理与修复.E-mail:mshuang@des.ecnu.edu.cn

中图分类号: X522
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- 文章访问数: 168
- HTML全文浏览量: 63
- PDF下载量: 224
- 被引次数: 0
出版历程
- 收稿日期: 2018-06-08
- 刊出日期: 2018-11-25

Screening of heterotrophic nitrification-aerobic denitrifying bacteria and its nitrogen removal characteristics

ZHANG Ting-yue^{1,2
,},
DING Yu^1,2,
HUANG Min-sheng^{1,2
, ,}

1.
School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China
2.
Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai 200241, China

摘要

摘要: 为寻求高效水体脱氮手段，从龙泓涧梯级塘底泥中筛选出以Pseudomonas菌属为主、具有异养硝化-好氧反硝化功能菌群，将其命名为LHJ-1.异养硝化和好氧反硝化性能研究结果表明，菌群LHJ-1具有明显的异养硝化功能，对NH₄⁺-N和TOC利用率分别达99.90%和56.69%，且表现出较高的反硝化能力，对NO₃^--N和NO₂^--N的转化率分别为92.46%和89.67%.由不同环境因素（碳氮比、碳源、pH值和溶解氧）影响实验可知，多种环境因子均对菌群LHJ-1脱氮效果具有较大影响，因此在实际应用中需考察不同环境因子，以找出最佳生长条件，获得最大脱氮效率.异养硝化-好氧反硝化菌群LHJ-1的筛选在水体脱氮除碳中具有广阔的应用前景.
- 异养硝化 /
- 好氧反硝化 /
- 混合菌 /
- 脱氮性能
Abstract: In order to find a high-efficiency denitrification method, a Pseudomonas genus-based strain was selected from the sediment of Cascade Pond in Longhong Ravine. The strain had heterotrophic nitrification and aerobic denitrification capability and was named LHJ-1. The results showed that the bacterial consortium LHJ-1 had clear heterotrophic nitrification capability; the utilization rates of NH₄⁺-N and TOC were 99.90% and 56.69%, respectively. Concurrently, it demonstrated good denitrification ability, with conversion rates of NO₃^--N and NO₂^--N at 92.46% and 89.67%, respectively. Experiments evaluating environmental effects showed that various factors (e.g., C/N ratio, carbon source, pH, and DO concentration) could have a significant influence on the denitrification effect. Accordingly, different environmental factors should be investigated in practical applications to determine the optimum growth conditions for the best nitrogen removal efficiency. The screening of heterotrophic nitrification and aerobic denitrifying bacteria consortium LHJ-1 has broad application prospects for both denitrification and decarbonization of water.
- heterotrophic nitrification /
- aerobic denitrification /
- mixed bacterial community /
- denitrification characteristics

HTML全文

图 1 菌群LHJ-1属水平群落丰度组成

Fig. 1 Abundance of bacterial consortium LHJ-1 community at the Genus level

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图 2 菌群LHJ-1异养硝化特性

Fig. 2 Heterotrophic nitrification characteristics of bacterial consortium LHJ-1

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图 3 以NO₃^--N (a)和NO₂^--N (b)为氮源时菌群LHJ-1好氧反硝化特性

Fig. 3 Aerobic denitrification characteristics of bacterial consortium LHJ-1 with NO₃^--N(a) and NO₂^--N (b) as the nitrogen sources

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图 4 不同C/N下菌群LHJ-1脱氮特性

Fig. 4 Nitrogen removal characteristics of bacterial consortium LHJ-1 with different C/N ratios

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图 5 不同碳源下菌群LHJ-1脱氮特性

Fig. 5 Nitrogen removal characteristics of bacterial consortium LHJ-1 with different carbon sources

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图 6 不同pH值下菌群LHJ-1脱氮特性

Fig. 6 Nitrogen removal characteristics of bacterial consortium LHJ-1 with different pH values

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图 7 不同DO浓度下菌群LHJ-1脱氮特性

Fig. 7 Nitrogen removal characteristics of bacterial consortium LHJ-1 with different dissolved oxygen concentrations

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参考文献(35)

[1]	杨垒.高效异养硝化细菌的脱氮特性及其处理高氨氮废水研究[D].西安: 西安建筑科技大学, 2016. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=D01059279
[2]	刘天琪.异养硝化-好氧反硝化菌ADN-42的脱氮特性[D].辽宁大连: 大连理工大学, 2014. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hjwrzljsysb201502080
[3]	FU Z M, YANG F L, AN Y Y, et al. Simultaneous nitrification and denitrification coupled with phosphorus removal in an modified anoxic/oxic-membrane bioreactor (A/O-MBR)[J]. Biochemical Engineering Journal, 2009, 43(2): 191-196. doi: 10.1016/j.bej.2008.09.021
[4]	JI Z Y, CHEN Y G. Using sludge fermentation liquid to improve wastewater short-cut nitrification-denitrification and denitrifying phosphorus removal via nitrite[J]. Environmental Science & Technology, 2010, 44(23): 8957-8963. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=173aeb536e179386ddc96dec4b005df4
[5]	CHOI K, ZHANG S, SONG J H, et al. Aerobic denitrification by a heterotrophic nitrifying-aerobic denitrifying (HN-AD) culture enriched activated sludge[J]. KSCE Journal of Civil Engineering, 2017, 21(6): 2113-2118. doi: 10.1007/s12205-016-1287-6
[6]	QIAO S, LIU X J, ZHOU J T. Research progress of heterotrophic nitrification-aerobic denitrification in biological denitrification[J]. Journal of Safety & Environment, 2014, 14(2): 128-135. http://d.old.wanfangdata.com.cn/Periodical/aqyhjxb201402028
[7]	黄廷林, 赵金亚, 张海涵.一株异养硝化-好氧反硝化菌JY78的筛选及其脱氮特性研究[J].安全与环境学报, 2016, 16(4): 325-331. http://d.old.wanfangdata.com.cn/Periodical/aqyhjxb201604066
[8]	ROBERTSON L A, KUENEN J G. Aerobic denitrification: A controversy revived[J]. Archives of Microbiology, 1984, 139(4): 351-354. doi: 10.1007/BF00408378
[9]	JOO H S, HIRAI M, SHODA M. Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No.4[J]. Journal of Bioscience and Bioengineering, 2005, 100(2): 184-191. doi: 10.1263/jbb.100.184
[10]	ZHANG J B, WU P X, HAO B, et al. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001[J]. Bioresource Technology, 2011, 102(21): 9866-9869. doi: 10.1016/j.biortech.2011.07.118
[11]	ZHANG Q L, LIU Y, AI G M, et al. The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7[J]. Bioresource Technology, 2012, 108: 35-44. doi: 10.1016/j.biortech.2011.12.139
[12]	CHEN P Z, LI J, LI Q X, et al. Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24[J]. Bioresource Technology, 2012, 116: 266-270. doi: 10.1016/j.biortech.2012.02.050
[13]	王永刚, 王旭, 张俊娥, 等.好氧反硝化细菌研究及应用进展[J].工业水处理, 2017(2): 12-17. http://d.old.wanfangdata.com.cn/Periodical/gyscl201702003
[14]	马放, 王弘宇, 周丹丹.好氧反硝化生物脱氮机理分析及研究进展[J].工业用水与废水, 2005, 36(2): 4. doi: 10.3969/j.issn.1009-2455.2005.02.002
[15]	高廷耀, 周增炎.生物脱氮工艺中的同步硝化反硝化现象[J].给水排水, 1998(12): 6-9.
[16]	郭琳.水源水库中好氧反硝化菌的筛选及脱氮性能研究[D].西安: 西安建筑科技大学, 2015. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=D01059329
[17]	ZHU L, DING W, FENG L J, et al. Isolation of aerobic denitrifiers and characterization for their potential application in the bioremediation of oligotrophic ecosystem[J]. Bioresource Technology, 2012, 108: 1-7. doi: 10.1016/j.biortech.2011.12.033
[18]	杨霄.分层型水库水体细菌群落演变机制与扬水曝气强化作用研究[D].西安: 西安建筑科技大学, 2016. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=D01059275
[19]	梁贤.一株异养硝化细菌YL的筛选及其脱氮特性研究[D].西安: 西安建筑科技大学, 2015. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=D714072
[20]	康鹏亮, 张海涵, 黄廷林, 等.湖库沉积物好氧反硝化菌群脱氮特性及种群结构[J].环境科学, 2018(5): 2431-2437. http://d.old.wanfangdata.com.cn/Periodical/hjkx201805052
[21]	苏兵.一株海洋异养硝化—好氧反硝化菌的分离鉴定、脱氮特性及应用[D].福建厦门: 集美大学, 2014. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y2631743
[22]	安强, 赵彬, 何义亮.异养硝化菌Alcaligenes faecalis strain NR的硝化性能及其酶活性[J].上海交通大学学报, 2012, 46(5): 774-779. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK201205297317
[23]	黄廷林, 张丽娜, 张海涵, 等.一株贫营养异养硝化-好氧反硝化菌的筛选及脱氮特性[J].生态环境学报, 2015(1): 113-120. http://d.old.wanfangdata.com.cn/Periodical/tryhj2015010018
[24]	田凤蓉, 张彬彬, 杨志林, 等.高效脱氨除臭异养硝化菌的筛选鉴定及脱氨性能研究[J].环境工程, 2014(5): 64-68. doi: 10.3969/j.issn.1671-1556.2014.05.011
[25]	HE T X, LI Z L, SUN Q, et al. Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion[J]. Bioresource Technology, 2016, 200: 493-499. doi: 10.1016/j.biortech.2015.10.064
[26]	王国英, 崔杰, 岳秀萍, 等.异养硝化-好氧反硝化菌脱氮同时降解苯酚特性[J].中国环境科学, 2015, 35(9): 2644-2649. doi: 10.3969/j.issn.1000-6923.2015.09.012
[27]	潘玉瑾, 刘芳, 孟爽, 等.好氧反硝化菌P.chengduensis ZPQ2的筛选及其反硝化条件优化[J].环境工程, 2016(1): 41-46. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hjgc201601009
[28]	林娜, 郭楚玲, 柯林, 等.富营养化池塘中好氧反硝化菌的分布及脱氮研究[J].中国科技论文在线, 2010(5): 369-372. doi: 10.3969/j.issn.2095-2783.2010.05.007
[29]	YINGCHIH C, MINGSHIUM C. Determination of optimal COD/nitrate ratio for biological denitrification[J]. International Biodeterioration & Biodegradation, 2003, 51(1): 43-49. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=850682b9017bcb91677172c55084df97
[30]	LI C, YANG J, WANG X, et al. Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24[J]. Bioresource Technology, 2015, 182: 18-25. doi: 10.1016/j.biortech.2015.01.100
[31]	颜薇芝, 张汉强, 余从田, 等. 1株异养硝化好氧反硝化不动杆菌的分离及脱氮性能[J].环境工程学报, 2017(7): 4419-4428. http://d.old.wanfangdata.com.cn/Periodical/hjwrzljsysb201707073
[32]	蔡茜, 何腾霞, 冶青, 等.耐冷嗜碱蒙氏假单胞菌H97的鉴定及其好氧反硝化特性[J].环境科学, 2018(7): 1-15. doi: 10.3969/j.issn.1673-1212.2018.07.001
[33]	YANG L, REN Y X, LIANG X, et al. Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous wastewater[J]. Bioresource Technology, 2015, 193: 227-233. doi: 10.1016/j.biortech.2015.05.075
[34]	马放, 王弘宇, 刘锋刚.溶解氧和有机碳源对同步硝化好氧反硝化脱氮的影响[C]//全国环境微生物学学术研讨会.第八次全国环境生物学学术研讨会论文集, 北京: 化学工业出版社, 2005. http://www.wanfangdata.com.cn/details/detail.do?_type=conference&id=6097213
[35]	孙移鹿, 张雪宁, 李昂.基于溶解氧调控缓解好氧反硝化过程亚硝酸盐积累的研究[C]//全国环境微生物学学术研讨会.第十七次全国环境生物学学术研讨会论文集, 北京: 化学工业出版社, 2014. http://www.wanfangdata.com.cn/details/detail.do?_type=conference&id=8505777