河道修复中常见植物种类对淡色库蚊产卵行为的影响

肖冰; 马明海; 黄民生; 陈奇; 冷培恩; 何岩

doi:10.3969/j.issn.1000-5641.2019.06.013

河道修复中常见植物种类对淡色库蚊产卵行为的影响

doi: 10.3969/j.issn.1000-5641.2019.06.013

肖冰^1,2,,
马明海^1,2,3,
黄民生^1,2, ,,
陈奇^1,2,
冷培恩⁴,
何岩^1,2

1.
华东师范大学生态与环境科学学院, 上海 200241
2.
华东师范大学上海市城市化生态过程与生态恢复重点实验室, 上海 200241
3.
黄山学院生命与环境科学学院, 安徽黄山 245041
4.
上海市疾病预防控制中心病媒生物防治科, 上海 200336

基金项目:

国家自然科学基金 51278192

国家科技重大专项 2013ZX07101012

普陀区高层次人才科研创新项目普人才2014-A-18

详细信息

作者简介:
肖冰, 女, 硕士研究生, 研究方向为城市水环境与蚊虫孳生.E-mail:xiaobing_19940430@163.com

通讯作者:
黄民生, 男, 教授, 博士生导师, 研究方向为水环境治理与修复.E-mail:mshuang@des.ecnu.edu.cn

中图分类号: Q965
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出版历程
- 收稿日期: 2018-08-07
- 刊出日期: 2019-11-25

Ovipositional response of Culex pipiens pallens to common species of plants used in river ecological restoration

XIAO Bing^{1,2
,},
MA Ming-hai^1,2,3,
HUANG Min-sheng^{1,2
, ,},
CHEN Qi^1,2,
LENG Pei-en⁴,
HE Yan^1,2

1.
School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China
2.
Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai 200241, China
3.
School of Life and Environmental Sciences, HuangShan University, Huangshan Anhui 245041, China
4.
Department of Vector Control, Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China

摘要

摘要: 比较研究了石菖蒲、香菇草、鱼腥草、粉绿狐尾藻和绿薄荷5种植物及其挥发性物质对淡色库蚊产卵行为的影响.结果表明，石菖蒲的蚊虫产卵活性指数（OAI）值为+0.58，抑制率为0%，具有较强的引诱作用；香菇草、鱼腥草和粉绿狐尾藻的OAI值分别为-0.70、-0.64和-0.16，抑制率分别为82.46%、78.36%和27.05%，其中香菇草和鱼腥草的OAI值均小于-0.30，可作为抑制剂；5种植物中，绿薄荷的抑制作用最强（OAI=-1.00，ER=100.00%），最适合作为驱蚊植物.挥发性有机物中苯类化合物与OAI值呈显著正相关（r=0.09，p < 0.05），萜烯类中的单萜烯类与OAI值呈显著负相关（r=-0.09，p < 0.05）.本文为河道修复及蚊虫防治的协同发展提供了理论依据.
- 植物化学物质 /
- 淡色库蚊 /
- 产卵选择性
Abstract: This study tested the ovipositional response of Cx. pipiens pallens to five species of plants and their volatiles:Acorus tatarinowii, Hydrocotyle vulgaris, Houttuynia cordata Thunb, Myriophyllum aquaticum (Vell.) Verdc. and Mentha spicata. The results showed that the oviposition activity index (OAI) value of Acorus tatarinowii was +0.58 with an effective repellency (ER) of 0%; Hence, Acorus tatarinowii can be considered as an oviposition attractant/stimulant. The OAI of Hydrocotyle vulgaris, Houttuynia cordata Thunb, and Myriophyllum aquaticum (Vell.) Verdc were measured as -0.70, -0.64 and -0.16 with an ER of 82.46%, 78.36% and 27.05%, respectively. Among these, Hydrocotyle vulgaris and Houttuynia cordata Thunb can be considered as repellents/deterrents. Meanwhile, the OAI of Mentha spicata was -1.00 with an ER of 100%, i.e., the strongest inhibiting effect, and hence considered the most suitable plant for repelling mosquitos. According to correlation analyses, there were significantly negative correlations between benzenoids and OAI values (p < 0.05). Terpenes, especially monoterpenes, had a significant positive correlation with OAI values (p < 0.05). This study provides a basis for the synergetic development of river ecological restoration and mosquito control.
- phytochemicals /
- Culex pipiens pallens /
- ovipositional selection

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图 1 蚊虫嗅觉系统在产卵选择行为中的调控^[12]

Fig. 1 Oviposition site selection behavior of the mosquito olfactory system

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图 2 成蚊产卵试验装置

Fig. 2 Apparatus for oviposition response testing of adult mosquitoes

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图 3 种植物总离子流

注: a石菖蒲Acorus tatarinowii; b香菇草Hydrocotyle vulgaris; c鱼腥草Houttuynia cordata Thunb; d粉绿狐尾藻Myriophyllum aquaticum (Vell.) Verdc; e绿薄荷Mentha spicata

Fig. 3 Total ion current of five plants

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表 1 植物鲜重

Tab. 1 Fresh weight of plants

植物	鲜重/g
石菖蒲	2.947
香菇草	2.913
鱼腥草	2.315
粉绿狐尾藻	2.587
绿薄荷	2.027

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表 2 淡色库蚊在不同植物条件下的产卵反应

Tab. 2 Ovipositional response of Culex pipiens pallens to five different plants

植物	产卵数^*	OAI	ER
石菖蒲	465.00± 164.59	+0.58	0
香菇草	21.40± 13.38	-0.70	82.46%
鱼腥草	26.40± 20.53	-0.64	78.36%
粉绿狐尾藻	89.00± 37.88	-0.16	27.05%
绿薄荷	0.00± 0.00	-1.00	100.00%
注:*产卵数为平均值±标准差, 数量n=5

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表 3 5种植物挥发物中主要成分的相对含量及与OAI值的相关性

Tab. 3 Relative composition of major chemical components from volatiles of the five plants and its correlation with OAI values

挥发性有机物种类		化合物的相对含量					相关系数
挥发性有机物种类		石菖蒲^a/%	香菇草^a/%	鱼腥草^a/%	粉绿狐尾藻^a/%	绿薄荷^a/%	相关系数
酯类		0.67	-	0.02	4.12	0.18	0.60
酚类		0.51	-	0.17	3.28	-	0.87
醇类		2.41	0.02	28.04	-	6.82	-0.30
酮类		0.43	-	5.94	2.04	25.07	-0.40
醛类		0.09	-	-	1.87	-	0.78
醚类		1.68	-	4.07	2.17	-	0.56
链状烷烃		1.03	26.76	0.24	27.00	-	0.50
其他烯烃		13.03	20.61	12.38	18.93	1.70	0.30
苯类		0.41	0.12	0.39	3.13	-	0.90^*
酸类		-	-	-	0.18	-	0.35
萜烯类	单萜烯	1.53	5.77	3.13	-	45.15	-0.90^*
	倍半萜烯	32.68	42.98	33.24	-	20.20	-0.20
	小计	34.21	48.75	36.37	-	65.35	-0.90^*
注:^a表示5种植物中每种主要化合物成分的相对含量; *指在0.05水平上显著相关

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参考文献(31)

[1]	RANSON H, LISSENDEN N. Insecticide resistance in African anopheles mosquitoes:A worsening situation that needs urgent action to maintain malaria control[J]. Trends in Parasitology, 2016, 32(3):187-196. doi: 10.1016/j.pt.2015.11.010
[2]	RANSON H, N'GUESSAN R, LINES J, et al. Pyrethroid resistance in African anopheline mosquitoes:What are the implications for malaria control?[J]. Trends in Parasitology, 2011, 27(2):91-98. doi: 10.1016/j.pt.2010.08.004
[3]	MUEMA J M, BARGUL J L, NJERU S N, et al. Prospects for malaria control through manipulation of mosquito larval habitats and olfactory-mediated behavioural responses using plant-derived compounds[J]. Parasites & Vectors, 2017(10):184. http://cn.bing.com/academic/profile?id=1ef3f3a0e2985cebfdd1b7e4aaa69053&encoded=0&v=paper_preview&mkt=zh-cn
[4]	RUSSELL T L, GOVELLA N J, AZIZI S, et al. Increased proportions of outdoor feeding among residual malaria vector populations following increased use of insecticide-treated nets in rural Tanzania[J]. Malaria Journal, 2011(10):80. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Doaj000001153245
[5]	NEAFSEY D E, WATERHOUSE R M, ABAI M R, et al. Highly evolvable malaria vectors:Thegenomes of 16Anophelesmosquitoes[J]. Science, 2015, 347(6217):1258522. doi: 10.1126/science.1258522
[6]	RUSSELL T L, BEEBE N W, COOPER R D, et al. Successful malaria elimination strategies require interventions that target changing vector behaviours[J]. Malaria Journal, 2013(12):56. http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_3570334
[7]	NAVARRO-SILVA M A, MARQUES F A, DUQUE L J E. Review of semiochemicals that mediate the oviposition of mosquitoes:A possible sustainable tool for the control and monitoring of Culicidae[J]. Revista Brasileira de Entomologia, 2009, 53(1):1-6. doi: 10.1590/S0085-56262009000100002
[8]	MOLLER-JACOBS L L, MURDOCK C C, THOMAS M B. Capacity of mosquitoes to transmit malaria depends on larval environment[J]. Parasites & Vectors, 2014(7):593. http://cn.bing.com/academic/profile?id=48ba502a99afaeba220038f1761082ac&encoded=0&v=paper_preview&mkt=zh-cn
[9]	TAKKEN W, KNOLS B G J. Odor-mediated behavior of afrotropical malaria mosquitoes[J]. Annual Review of Entomology, 1999, 44(17):131-157. http://cn.bing.com/academic/profile?id=289855a0b6dfd7ff060c75feadb96eb6&encoded=0&v=paper_preview&mkt=zh-cn
[10]	WANG G R, CAREY A F., CARLSON J R, et al. Molecular basis of odor coding in the malaria vector mosquito Anopheles gambiae[J]. Proc Natl Acad Sci USA, 2010, 107(9):4418-4423. doi: 10.1073/pnas.0913392107
[11]	TUMLINSON J H. The importance of volatile organic compounds in ecosystem functioning[J]. Journal of Chemical Ecology, 2014, 40(3):212. doi: 10.1007/s10886-014-0399-z
[12]	HERRERA-VARELA M, LINDH J, LINDSAY S W, et al. Habitat discrimination by gravid Anopheles gambiae sensu lato-a push-pull system[J]. Malaria Journal, 2014, 13:133. doi: 10.1186/1475-2875-13-133
[13]	MA M H, HUANG M S, LENG P E. Abundance and distribution of immature mosquitoes in urban riversproximate to their larval habitats[J]. Acta Tropica, 2016, 162:121-129. http://cn.bing.com/academic/profile?id=231905e57e7fbb6d793d38e9c5ba8bff&encoded=0&v=paper_preview&mkt=zh-cn
[14]	RAJKUMAR S, JEBANESAN A. Larvicidal and oviposition activity of Cassia obtusifolia Linn (Family:Leguminosae) leaf extract against malarial vector, Anopheles stephensi Liston (Diptera:Culicidae)[J]. Parasitology Research, 2009, 104(2):337-340. doi: 10.1007/s00436-008-1197-8
[15]	GOVINDARAJAN M, MATHIVANAN T, ELUMALAI K, et al. Ovicidal and repellent activities of botanical extracts against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi (Diptera:Culicidae)[J]. Asian Pacific Journal of Tropical Biomedicine, 2011, 1(1):43-48. doi: 10.1016/S2221-1691(11)60066-X
[16]	黄民生, 马明海, 曹承进, 等.城市水体环境及其治理:案例分析[M].北京:中国建筑工业出版社, 2017.
[17]	史睿杰, 谢寿安, 赵薇, 等.青海云杉针叶和枝条的挥发性化合物的固相微萃GC/MS分析[J].西北林学院学报, 2011, 26(6):95-99. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=xblxyxb201106021
[18]	GANESAN K, MENDKI M J, SURYANARAYANA M V S, et al. Studies of Aedes aegypti (Diptera:Culicidae) ovipositional responses to newly identified semiochemicals from conspecific eggs[J]. Australian Journal of Entomology, 2006, 45:75-80. doi: 10.1111/j.1440-6055.2006.00513.x
[19]	KRAMER W L, MULLA M S. Oviposition attractants and repellents of mosquitoes:Oviposition responses of culex mosquitoes to organic infusions[J]. Environmental Entomology, 1979, 8(6):1111-1117. doi: 10.1093/ee/8.6.1111
[20]	GAO Y, JIN Y J, LI H D, et al. Volatile organic compounds and their roles in bacteriostasisin five conifer Species[J]. Journal of Integrative Plant Biology, 2005, 47(4):499-507. http://cn.bing.com/academic/profile?id=8e3737b6b89a3dc08f75f198c2532342&encoded=0&v=paper_preview&mkt=zh-cn
[21]	GOVINDARAJAN M, SIVAKUMAR R, RAJESWARI M, et al. Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species[J]. Parasitology Research, 2012, 110(5):2023-2032. doi: 10.1007/s00436-011-2731-7
[22]	盛辛辛, 曹谨玲, 赵凤岐, 等.芦苇和美人蕉及薄荷用作人工湿地植物对中水的净化效果[J].湖南农业大学学报(自然科学版), 2013, 39(4):423-428. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hunannydx201304018
[23]	霍张丽, 朱广龙, 张江汀, 等.模拟人工湿地植物对富营养化水体的修复研究[J].水土保持研究, 2014, 21(5):267-271. http://d.old.wanfangdata.com.cn/Thesis/D495463
[24]	BAAK-BAAK C M, RODRÍGUEZ-RAMÍREZ A D, GARCÍA-REJÓN J E, et al. Development and laboratory evaluation of chemically-based baited ovitrap for the monitoring of Aedes aegypti[J]. Journal of Vector Ecology, 2013, 38(1):175-181. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=c1198706eb35cd8072f05b5d6fabf4f4
[25]	MATASYOH J C, WATHUTA E M, KARIUKI S T, et al. Chemical composition and larvicidal activity of Piper capense essential oil against the malaria vector, Anopheles gambiae[J]. Journal of Asia-Pacific Entomology, 2011, 14(1):26-28. doi: 10.1016/j.aspen.2010.11.005
[26]	AUTRAN E S, NEVES I A, DA SILVA C S B, et al. Chemical composition, oviposition deterrent and larvicidal activities against Aedes aegypti of essential oils from Piper marginatum Jacq. (Piperaceae)[J]. Bioresource Technology, 2009, 100(7):2284-2288. doi: 10.1016/j.biortech.2008.10.055
[27]	PERRY A S, FAY R W. Correlation of chemical constitution and physical properties of fatty acid esters with oviposition response of Ae. aegypti[J]. Mosquito News, 1967, 27(2):175-183. https://www.researchgate.net/publication/284635677_Correlation_of_chemical_constitution_and_physical_properties_of_fatty_acid_esters_with_oviposition_response_of_Aedes_aegypti
[28]	SHARMA K R, SEENIVASAGAN T, RAO A N, et al. Oviposition responses of Aedes aegypti and Aedes albopictus to certain fatty acid esters[J]. Parasitology Research, 2008, 103(5):1065-1073. doi: 10.1007/s00436-008-1094-1
[29]	NYASEMBE V O, TORTO B. Volatile phytochemicals as mosquito semiochemicals[J]. Phytochemistry Letters, 2014, 8(1):196-201. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=f1c61d75e61e7483727b14365f37a7de
[30]	DU Y J, MILLAR J G. Electroantennogram and oviposition bioassay responses of culex quinquefasciatus and culex tarsalis (Diptera:Culicidae) to chemicals in odors from bermuda grass infusions[J]. Journal of Medical Entomology, 1999, 36(2):158-166. doi: 10.1093/jmedent/36.2.158
[31]	AFIFY A, GALIZIA C G. Gravid females of the mosquito Aedes aegypti avoid oviposition on m-cresol in the presence of the deterrent isomer p-cresol[J]. Parasites & Vectors, 2014(7):315. doi: 10.1186/1756-3305-7-315