Preparation and fluorescence detection properties of ZnO nanostructure based on microdroplets
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摘要: 基于液滴微反应器, 通过水热法合成 ZnO 纳米结构. 该芯片集成了多种功能单元, 包括用于液滴生成的T形通道, 液滴汇合的 Y 形通道, 以及快速混合和观察纳米结构形成的 S 形通道. 通过调节水相和油相的流量改变液滴的尺寸, 研究微液滴中制备的纳米结构的形貌和尺寸, 并利用硫氰酸荧光素标记的羊抗牛 IgG 研究其荧光检测性能. 该工作表明, 通过流体动力学耦合形成的微液滴可制备 ZnO 纳米结构, 其颗粒形貌和尺寸随着液滴尺寸的变化而改变. 加热温度为 75 ℃, 油相、氨水、锌盐溶液流量分别为600、30、90 μL/h时制备的ZnO纳米结构具有最优的荧光检测性能.Abstract: In this paper, we discuss ZnO nanostructures synthesized by a hydrothermal method in a droplet microreactor. The microfluidic chip used integrates a multi-function unit that includes a T-channel for droplet formation, a Y-channel for droplet fusion, and an S-channel for rapid mixing and observation of the nanoparticle formation process. By adjusting the flow rates of the aqueous phase and the oil phase, we studied the morphology and size of droplets; fluorescence detection of the ZnO nanostructure synthesized, moreover, was evaluated by an FITC-labeled goat anti-bovine IgG. This work shows that ZnO nanostructures can be prepared by fluid dynamic coupling of droplets and that the morphology and size of the nanostructures vary with droplet size. When the flow rates of the oil phase, ammonia water, and ferric solution were 600, 30, and 90 μL/h, respectively, the ZnO nanostructure synthesized at 75 °C showed optimal fluorescence detection performance.
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Key words:
- microfluidic chip /
- droplet /
- ZnO nanostructure /
- fluorescence detection
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图 2 微液滴技术制备纳米结构的流程图
Fig. 2 Process diagram for preparation of the nanostructure by microdroplet technology
图 3 ZnO纳米结构对FITC-anti bovine IgG的检测示意图
Fig. 3 Schematic diagram of a ZnO nanostructure for the detection of FITC-anti bovine IgG
图 4 不同油相流量条件下的液滴形成与融合过程
Fig. 4 The droplet formation and fusion process under different oil phase flow conditions
图 6 不同流量比条件下合成ZnO纳米结构的AFM(a)—(b)和SEM(f)—(j)图
注：油相流量为600 μL/h, 氨水的流量为30 μL/h, 氨水与锌盐溶液的流量比为1 : 1—1 : 5
Fig. 6 AFM (a)—(b)and SEM (f)—(j) of ZnO nanostructure synthesized under different flow rate ratios
图 8 ZnO纳米结构XRD(a), EDS(b)图
Fig. 8 XRD pattern（a）and EDS （b） diagram of ZnO nanomaterials
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