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纳米银对河口潮滩硝酸盐异化还原成铵过程的影响

张梦霞 郑艳玲 尹国宇 董宏坡 韩平 高娟 刘程 常永凯 刘敏 侯立军

张梦霞, 郑艳玲, 尹国宇, 董宏坡, 韩平, 高娟, 刘程, 常永凯, 刘敏, 侯立军. 纳米银对河口潮滩硝酸盐异化还原成铵过程的影响[J]. 华东师范大学学报(自然科学版), 2020, (3): 68-77. doi: 10.3969/j.issn.1000-5641.201941005
引用本文: 张梦霞, 郑艳玲, 尹国宇, 董宏坡, 韩平, 高娟, 刘程, 常永凯, 刘敏, 侯立军. 纳米银对河口潮滩硝酸盐异化还原成铵过程的影响[J]. 华东师范大学学报(自然科学版), 2020, (3): 68-77. doi: 10.3969/j.issn.1000-5641.201941005
ZHANG Mengxia, ZHENG Yanling, YIN Guoyu, DONG Hongpo, HAN Ping, GAO Juan, LIU Cheng, CHANG Yongkai, LIU Min, HOU Lijun. Effects of silver nanoparticles on dissimilatory nitrate reduction in estuarine and tidal sediments[J]. Journal of East China Normal University (Natural Sciences), 2020, (3): 68-77. doi: 10.3969/j.issn.1000-5641.201941005
Citation: ZHANG Mengxia, ZHENG Yanling, YIN Guoyu, DONG Hongpo, HAN Ping, GAO Juan, LIU Cheng, CHANG Yongkai, LIU Min, HOU Lijun. Effects of silver nanoparticles on dissimilatory nitrate reduction in estuarine and tidal sediments[J]. Journal of East China Normal University (Natural Sciences), 2020, (3): 68-77. doi: 10.3969/j.issn.1000-5641.201941005

纳米银对河口潮滩硝酸盐异化还原成铵过程的影响

doi: 10.3969/j.issn.1000-5641.201941005
基金项目: 国家重点研发计划(2016YFA0600904); 国家自然科学基金(41725002, 41671463, 41130525, 41322002, 41601530)
详细信息
    通讯作者:

    侯立军, 男, 教授, 博士生导师, 研究方向为河口海岸生源要素循环. E-mail: ljhou@sklec.ecnu.edu.cn

  • 中图分类号: X171.1

Effects of silver nanoparticles on dissimilatory nitrate reduction in estuarine and tidal sediments

  • 摘要: 人类活动会导致纳米银(AgNPs)毒性污染物在河口海岸环境富集, 但AgNPs赋存和累积对河口氮转化过程的影响尚不清楚. 为此, 以长江口作为研究区域, 对不同粒径(10 nm、30 nm和100 nm)及不同浓度(0.1 mg/L、5 mg/L和10 mg/L) 的AgNPs进行暴露实验, 探究AgNPs对河口潮滩硝酸盐异化还原成铵(DNRA)的影响. 结果表明, 添加AgNPs对不同盐度沉积物DNRA速率均产生一定程度的抑制效应, 但其抑制率并没有随时间增长而明显增大. 受沉积物理化性质的影响, AgNPs对中盐度(8.0‰)沉积物DNRA速率抑制效应总体上高于其余盐度沉积物. 沉积物环境中AgNPs的粒径及浓度均是影响其毒性的重要因素: 当浓度不超过5 mg/L时, 10 nm粒径AgNPs毒性大于30 nm和100 nm粒径, 其在不同盐度沉积物中抑制率最高达16.03%、20.27%和15.36%; 但当AgNPs浓度为10 mg/L时, 30 nm和100 nm粒径的AgNPs对DNRA速率抑制程度明显增大, 毒性效应大于10 nm粒径AgNPs, 不同盐度沉积物中最大抑制率分别为17.48%、33.18%和26.45%. AgNPs释放的Ag+ 浓度与DNRA速率的抑制率未存在显著的正相关关系(p > 0.05), 反映AgNPs释放的Ag+对DNRA存在一定的抑制作用, 但并不能完全解释AgNPs的毒性作用特征. 研究结果对于客观评价金属纳米材料对河口氮循环的潜在影响具有重要科学意义.
  • 图  1  长江口潮滩沉积物采样站位

    Fig.  1  Map of the Yangtze Estuary showing the sampling sites

    图  2  培养期间AgNPs释放的Ag+浓度

    注: 误差棒代表标准偏差

    Fig.  2  Release of soluble silver from AgNPs in LHK, DHNC, and JS during incubation

    图  3  培养期间表层沉积物理化因子变化

    注: KB表示不添加AgNPs的样品, 误差棒代表标准偏差

    Fig.  3  Change of physicochemical factors in LHK, DHNC, and JS sediment during incubation

    图  4  AgNPs对沉积物DNRA速率的影响

    注: DNRA速率变化率为添加AgNPs组和空白组速率之间的差值与空白组速率的比值; KB表示空白组, 10 nm、30 nm和100 nm分别表示不同AgNPs粒径, 0.1 mg/L、30 mg/L和100 mg/L分别为不同AgNPs浓度; 误差棒代表标准偏差

    Fig.  4  Effect of AgNPs on DNRA rates

    表  1  沉积物DNRA速率与环境因子的相关性分析

    Tab.  1  Correlation analyses between environmental factors and DNRA rates in the sediment

    粒径NH4+NO3NO2TOC硫化物
    相关系数0.0030.0180.2090.0110.1740.105
    p0.7670.4800.0110.5880.0220.013
    下载: 导出CSV

    表  2  AgNPs粒径、AgNPs浓度和沉积物盐度对DNRA速率变化率的多因素方差分析

    Tab.  2  Multivariate ANOVA analyses of percentage changes in DNRA rates with AgNPs sizes, AgNPs concentration, and sediment salinity

    自由度df均方MSFp
    AgNPs 粒径217.8062.6650.043
    沉积物盐度259.6993.0030.054
    AgNPs 浓度2976.93330.9410.011
    AgNPs 粒径 × 盐度44.2520.2140.930
    AgNPs 粒径 × AgNPs 浓度472.2463.6340.008
    AgNPs 浓度 × 盐度459.1632.9760.022
    AgNPs 粒径 × 盐度 × AgNPs 浓度810.1620.5110.846
    下载: 导出CSV
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出版历程
  • 收稿日期:  2019-02-01
  • 录用日期:  2019-03-11
  • 网络出版日期:  2020-05-29
  • 刊出日期:  2020-05-01

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