Response of nitrogen removal in the overlying water to sediment resuspension in the intertidal wetlands of the Yangtze Estuary
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摘要: 以长江口潮滩作为研究区域, 采用15N同位素示踪技术, 模拟研究了沉积物再悬浮过程对水体反硝化和厌氧氨氧化的影响. 结果表明, 沉积物再悬浮引起的上覆水体反硝化和厌氧氨氧化速率与水体浊度呈显著的正相关关系, 这说明沉积物再悬浮能够促进水体脱氮过程的发生. 在沉积物再悬浮条件下, 采样点反硝化与厌氧氨氧化速率受不同站位理化因素的影响, 存在明显的空间差异, 且主要受沉积物总有机碳含量的控制. 此外, 随着沉积物再悬浮浊度的增加, 水体中反硝化细菌nirS基因与厌氧氨氧化细菌16S rRNA基因丰度均呈增加趋势. 这说明沉积物再悬浮可增加水体脱氮功能菌群的丰度, 进而增加脱氮速率. 研究结果表明, 评价河口潮滩沉积物再悬浮对氮转化过程的影响具有重要的科学意义.Abstract: The 15N isotope tracer technique was used to examine the response of denitrification and anammox to sediment resuspension in the intertidal wetlands of the Yangtze Estuary. The results showed that both denitrification and anammox rates were positively correlated with the turbidity of the overlying water, which demonstrates that sediment resuspension can promote the occurrence of nitrogen removal processes in overlying water. The denitrification and anammox rates showed significant spatial differences due to the physiochemical characteristics at different sites under sediment resuspension and was mainly attributed to the sediment TOC contents. In addition, the abundance of nirS and anammox bacterial 16S rRNA genes increased with suspended sediment turbidity, which indicates that sediment resuspension could increase the abundance of nitrogen removal functional bacteria, thus promoting nitrogen removal rates. These results indicate that sediment resuspension has important influence on nitrogen transformation processes and facilitates nitrogen removal in intertidal wetlands.
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Key words:
- resuspension /
- turbidity /
- denitrification /
- anammox
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表 1 采样点理化性质及微生物组成
Tab. 1 Physicochemical properties and microbial composition of sampling sites
采样点 盐度/
psupH 平均粒径/
μmFe2+/
(mg·g–1)Fe3+/
(mg·g–1)TOC/
(mg·g–1)硫化物/
(μg·g–1)NH4+/
(μmol·g–1)NO3–/
(μmol·g–1)NO2–/
(μmol·g–1)nirS/
(copies·g–1)16S rRNA/
(copies·g–1)XP 0.10 7.83 15.20 0.36 0.29 6.00 0.06 1.85 110.89 0.06 1.96×107 1.54×106 LHK 0.10 7.58 17.20 0.25 0.39 4.98 0.58 0.03 200.84 0.11 6.49×107 4.87×106 SDK 0.20 7.73 22.96 0.20 0.37 11.97 3.00 0.04 135.17 0.08 9.28×107 8.33×106 BLG 0.10 8.13 19.30 0.15 0.30 6.39 0.48 0.51 290.38 0.07 7.16×106 4.13×105 DHNC 0.60 8.01 17.96 0.18 0.43 10.39 0.08 0.31 110.77 0.14 9.73×106 8.41E×105 LCG 0.80 8.17 12.21 0.20 0.41 4.79 0.87 1.63 127.83 0.07 3.83×107 5.12E×106 -
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