好氧反硝化菌的固定化及其效能研究

尹超; 李莹; 张婷月; 刘佳敏; 陈体达; 崔丹; 黄民生

doi:10.3969/j.issn.1000-5641.2021.04.001

好氧反硝化菌的固定化及其效能研究

doi: 10.3969/j.issn.1000-5641.2021.04.001

尹超^{1, 2, 3, 4,},
李莹^{1, 2, 3, 4},
张婷月^{1, 2, 3, 4},
刘佳敏^{1, 2, 3, 4},
陈体达^{1, 2, 3, 4},
崔丹^{1, 2, 3, 4},
黄民生^{1, 2, 3, 4, ,}

1.
华东师范大学生态与环境科学学院上海城市化生态过程与生态恢复重点实验室, 上海　200241
2.
崇明生态研究院, 上海　202162
3.
上海有机固废生物转化工程技术研究中心, 上海　200241
4.
自然资源部大都市区国土空间生态修复工程技术创新中心, 上海　200062

基金项目: 国家科技重大专项(2017ZX07207001, 2018ZX07208008)

详细信息

作者简介:
尹超：尹　超, 男, 博士研究生, 研究方向为水环境治理与修复. E-mail: yinchao31@163.com

通讯作者:
黄民生, 男, 教授, 博士生导师, 研究方向为水环境治理与修复. E-mail: mshuang@des.ecnu.edu.cn

中图分类号: X522
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- 文章访问数: 68
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- 被引次数: 0
出版历程
- 收稿日期: 2020-11-16
- 刊出日期: 2021-07-25

Immobilization and efficacy of an aerobic denitrifier

YIN Chao^{1, 2, 3, 4
,},
LI Ying^{1, 2, 3, 4},
ZHANG Tingyue^{1, 2, 3, 4},
LIU Jiamin^{1, 2, 3, 4},
CHEN Tida^{1, 2, 3, 4},
CUI Dan^{1, 2, 3, 4},
HUANG Minsheng^{1, 2, 3, 4
, ,}

1.
Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai　200241, China
2.
Institute of Eco-Chongming, Shanghai　202162, China
3.
Shanghai Engineering Rearch Center of Biotransformation of Organic Solid Waste, Shanghai　200241, China
4.
Technology Innovation Center for Land Spatial Eco-Restoration in Metropolitan Area (Ministry of Natural Resources), Shanghai　200062, China

摘要

摘要: 为了提高好氧反硝化菌的环境耐受性和脱氮效率, 采用聚乙烯醇(PVA)、海藻酸钠(SA)和稻壳粉作为载体对好氧反硝化菌进行固定化, 并对固定化颗粒的性能进行评价. 结果如下: 固定化颗粒最佳配比为12%聚乙烯醇(PVA)、8%海藻酸钠(SA)、0.5 g稻壳粉和10 mL菌液; 固定化颗粒具有较好的稳定性和传质性, 48 h的总氮(TN)去除率为89.35% ~ 90.12%. 固定化颗粒对pH值和转速具有良好的耐受性, pH值为11时, TN去除率为90%; 120 r/min时TN和NH₄⁺-N去除率最高, 分别为91.29%和93.30%; 固定化颗粒不耐低温(10℃和15℃), 在10℃时, TN去除率仅为20%左右; 但是在30℃时, TN去除率可达90.59%.
- 好氧反硝化菌 /
- 固定化 /
- 脱氮
Abstract: To improve the environmental tolerance and nitrogen removal efficiency of an aerobic denitrifier, polyvinyl alcohol (PVA), sodium alginate (SA), and rice hull powder were used as immobilized carriers for an aerobic denitrifier and the performance was subsequently evaluated. The results showed that the optimal ratio of immobilized particles was a mixture of 12% PVA, 8% sodium alginate (SA), 0.5 g rice hull powder, and 10 mL bacterial solution. The immobilized particles had strong stability and mass transfer capability; the removal efficiency of TN was 89.35% ~ 90.12% over 48h. The immobilized particles had good tolerance to pH and rotating speed. When the pH was 11, the removal efficiency of TN was 90%. The removal efficiency of TN and NH₄⁺-N was the highest (91.29% and 93.30%, respectively) when the speed was 120 r/min. The immobilized particles were not resistant to low temperatures (10℃ and 15℃), and the TN removal efficiency was only about 20% at 10℃. The TN removal efficiency, however, achieved 90.59% at 30℃.
- aerobic denitrifer /
- immobilization /
- denitrification

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图 1 不同转速对颗粒脱氮效果的影响

Fig. 1 Effect of different rotating speeds on the denitrification of pellets

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图 2 不同温度对颗粒脱氮效果的影响

Fig. 2 Effect of different temperatures on the denitrification of pellets

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图 3 不同pH值对颗粒脱氮效果的影响

Fig. 3 Effect of different pH levels on the denitrification of pellets

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表 1 载体材料对包埋颗粒成球情况影响

Tab. 1 The influence of carrier materials on pellet formation

影响因素	PVA浓度/(g·(100 mL)^–1)	SA浓度/(g·(100 mL)^–1)	稻壳粉量/(g·(100 mL)^–1)	菌液量/(mL·(100 mL)^–1)	颗粒成形情况
PVA浓度	12	0.1	1	10	拖尾, 表面有气泡
	10				粘连多
	8				有拖尾
SA浓度	10	0.1	1	10	粘连多
		0.5			较好
		1			较好
稻壳粉量	10	0.5	0.5	10	较好
			1		较好
			1.5		溶液色度大
菌液量	10	0.5	1	5	较好
				10	较好
				15	较好

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表 2 固定化载体配比优化正交试验

Tab. 2 Optimization orthogonal test of carrier

序号	因素				TN去除率/%
序号	A	B	C	D	TN去除率/%
1	8	0.4	0	5	76.29
2	8	0.6	0.5	10	79.02
3	8	0.8	1	15	78.28
4	9	0.4	0.5	15	76.52
5	9	0.6	1	5	77.39
6	9	0.8	0	10	73.30
7	10	0.4	1	10	77.65
8	10	0.6	0	15	75.1
9	10	0.8	0.5	5	78.46
注: 因素 A 为 PVA (%), 因素 B 为 SA(%), 因素 C 为稻壳粉(g), 因素 D 为菌液(mL); 表 3 同.

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表 3 正交试验数据处理结果

Tab. 3 The results of orthogonal test

	A	B	C	D
K₁_j	77.86	75.97	74.90	77.38
K₂_j	75.74	77.17	78.00	76.66
K₃_j	77.07	76.68	77.77	76.63
R	2.12	1.20	3.10	0.75
注: K₁_j、K₂_j、K₃_j代表各因素该水平下的平均值; R为极差, 极差越大, 说明该因素对指标影响越大.

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