Abstract: Nanomaterials are revolutionizing the development of sensors. Due to their quantum size effect and surface effect, the physical and other attributes of sensors can be improved by using nanomaterials. Moreover, application of nanostructured chemical or biosensors to their analyze multiple components in vivo is becoming available. Now, nanomaterial-based sensors have attracted considerable attention due to their excellent sensitivity, high selectivity and long-term stability. This review mainly focuses on the demonstration the nanomaterial-based electrochemical and biosensors developed by our research group. In addition, we also describe the progress on preparation and application of nanostructured sensors.