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摘要: 在实验生态条件下,研究了0号柴油分散液(WAFs)及加入溢油分散剂后的0号柴油乳化液(disWAFs)对马粪海胆〖WTBX〗(Hemicentrotus pulcherrimus)〖WTBZ〗的急性毒性作用;并研究了亚致死浓度下海胆消化腺中超氧化物歧化酶(SOD)、过氧化氢酶(CAT) 和谷胱甘肽硫转移酶(GST)活性的变化.结果表明:WAFs对马粪海胆幼胆的24,48,72和96 h半致死浓度分别为18.2,15.5,11.5和9.5 mg/L; disWAFs 对马粪海胆幼胆的24,48,72和96 h半致死浓度分别为11.7,9.1,7.4和5.1 mg/L.加入溢油分散剂后,使0号柴油分散液对马粪海胆的毒性效应变大.亚致死浓度下的disWAFs污染对消化腺内抗氧化酶活性的影响存在时间、剂量效应关系.同一剂量组随着污染时间的延长, SOD,CAT和GST的活性表现为先诱导后抑制的趋势,GST趋势尤其显著.受污染个体在污染解除之后, 其SOD,CAT和GST酶活性得到恢复.SOD,CAT和GST可以作为海洋环境石油烃污染监测的生物标志物,为生物损害评估提供依据.
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关键词:
- 马粪海胆 /
- 0号柴油分散液(WAFs) /
- 0号柴油乳化液(disWAFs) /
- 抗氧化酶
Abstract: The acute toxic effects of No.0 diesel oil wateraccomodated fractions(WAFs) and dispersed oil (disWAFs) on sea urchin were studied, and the response of antioxidase(superoxide dismutase, SOD; catalase, CAT; glutathione Stransferase,GST) in digestive gland to disWAFs were tested. Hemicentrotus pulcherrimus was exposed to different concentrations of disWAFs (0.5,1,2 mg/L) for 13 days, and then transferred into clean sea water for 11d restoring experiment. The results showed that the disWAFs was more toxic, with 24, 48, 72, 96h LC50 values of 11.7,9.1,7.4 and 5.1 mg/L respectively. The activity of antioxidase was statistically significant doserelated effect in different disWAFs concentrations. Under the same doseconcentration exposure, SOD, CAT and GST activities increased first and then decreased with the prolonged exposure time. This trend was most significant in GST activity. The activities of SOD, CAT and GST of all concentration groups recovered to the corresponding control level after recovery experiment. According to the data, SOD, CAT and GST can be taken as biomarkers to monitor benthic marine oil pollution and provide.
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