白蚁肠道木质素分解菌PY12的分离鉴定及<a>lip</a>基因的克隆

高云航; 王巍; 刘萌; 勾长龙; 娄玉杰; 马红霞

白蚁肠道木质素分解菌PY12的分离鉴定及lip基因的克隆

1.
吉林农业大学~~动物科技学院, 长春\;130118;
2.
吉林农业大学~~动物生产与产品质量安全教育部重点实验室,~~长春\;130118

详细信息

中图分类号: S182
计量
- 文章访问数: 1304
- HTML全文浏览量: 20
- PDF下载量: 1712
- 被引次数: 0
出版历程
- 收稿日期: 2013-10-01
- 修回日期: 2014-01-01
- 刊出日期: 2014-07-25

Isolation and identification of lignin decomposing bacteria and cloning of lip gene in termite gut

1.
College of Animal Science and Technology,\ Jilin Agricultural University, Changchun} 130118, China;
2.
Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Changchun 130118, China

摘要

摘要: 利用苯胺蓝平板法从白蚁肠道中分离到一株木质素分解高效菌株~PY~12, 经形态观察、生化鉴定和~16~S rRNA~鉴定为肠杆菌属的霍氏肠杆菌~(\textit{Enterobacter hormaechei}). 根据已报道的~$lip$~(木质素过氧化物酶, Lignin Peroxidase)~基因序列设计特异性引物, 克隆该菌的~$lip$~基因, 将其克隆入~pMD19-T~载体, 获得的核苷酸序列为~918~bp, 并与~GenBank~中已知的~$lip$~序列进行同源性对比, 同源性不高, 但该核苷酸翻译后的氨基酸序列比对结果发现, 其与假单胞杆菌~\textit{Pseudomonas} sp. 编码蛋白质的氨基酸序列同源性为88.5{\%}.
- 白蚁 /
- 木质素分解菌 /
- 16S rRNA /
- 木质素过氧化物酶基因
Abstract: A strain of bacterium that named PY12 was isolated from the termite gut using Azure B method, which had an efficiently lignin decomposition function. It was identificated as \textit{Enterobacter hormaechei}. by morphological, biochemical identification and\\ 16S rRNA identification. The $lip$ gene were amplified by the specific primers, and then cloning it into pMD19-T vector, the target fragment sequences were 918 bp, Homology comparison with the $lip$ gene which known in GenBank, the Homology was low, but when the nucleotide sequences were translated into protein, the homology was up to 88.5{\%} with the Pseudomonas sp..
- Termite /
- lignin decomposing bacteria /
- 16S rRNA /
- lignin peroxidase gene

HTML全文

参考文献(1)

[1]

{1} CARMEN S\'{A}NCHEZ. Lignocellulosic residues: Biodegradation and

bioconversion by fungi [J]. Biotechnol Adv, 2009, 27: 185-194.
{2} FENG C L, ZENG G M, HUANG D L, et al. Effect of ligninolytic enzymes on lignin degradation and carbon utilization during lignocellulosic waste composting [J]. Process Biochemistry, 2011, 46: 1515-1520.
{3} RAKESH K S, DALJIT S A. Production of lignocellulolytic enzymes and enhancement of in vitro digestibility during solid state fermentation of wheat straw by \textit{Phlebia floridensis} [J]. Bioresource Technology, 2010, 101: 9248-9253.
{4} 吴薇, 田世杰, 顿宝庆, 等. 高效木质素酶产生菌的分离筛选~[J]. 食品科技, 2010, 35(1): 10-14.
{5} 晋果果, 翁海波, 李萍, 等. 高温木质素降解菌~\textit{Geobacillus caldoxylosilyticus} J16~的筛选及其产酶发酵性质研究~[J]. 中国农学通报, 2011, 27(8): 334-339.

{6} JING K, DHRUBOJYOTI D L, CHEN S L. Varied lignin disruption mechanisms for different biomass substrates in lower termite [J]. Renewable Energy, 2013, 50: 1060-1064.
{7} TOKUDA G, LO N, WATANABE H. Marked variations in patterns of cellulase activity against crystalline-vs. carboxymethyl-cellulose in the digestive systems of diverse, wood-feeding termites [J]. Physiol Entomol, 2005, 30(4): 372-380.
{8} 周刚. 白蚁内生菌的分离及其纤维素酶、木质素酶高产菌株的鉴定~[D]. 沈阳: 黑龙江大学, 2006.

{9} 高云航, 王巍, 李秋菊, 等. 白蚁肠道木质素及纤维素分解菌的分离鉴定及产酶条件优化~[J]. 中山大学学报: 自然科学版, 2013, 52(2): 84-89.
{10} 姚火春. 兽医微生物学实验指导~[M]. 北京: 中国农业出版社, 2002: 36-42.
{11} 张桂山. 吉林白鹅肠道纤维分解菌分离鉴定的研究~[D]. 长春: 吉林农业大学, 2007: 9-21.

施引文献

资源附件(0)

访问统计