Responses of bacterial community structure in malodorous river sediments to aeration by DGGE

HE Yan; YAO Li-ping; LI Wen-chao; HUANG Min-sheng; ZHANG Yi-fan

doi:10.3969/j.issn.1000-5641.2015.02.010

Issue 2

May 2015

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2015 > (2): 84-90

HE Yan, YAO Li-ping, LI Wen-chao, HUANG Min-sheng, ZHANG Yi-fan. Responses of bacterial community structure in malodorous river sediments to aeration by DGGE[J]. Journal of East China Normal University (Natural Sciences), 2015, (2): 84-90. doi: 10.3969/j.issn.1000-5641.2015.02.010

Citation:

HE Yan, YAO Li-ping, LI Wen-chao, HUANG Min-sheng, ZHANG Yi-fan. Responses of bacterial community structure in malodorous river sediments to aeration by DGGE[J]. Journal of East China Normal University (Natural Sciences), 2015, (2): 84-90. doi: 10.3969/j.issn.1000-5641.2015.02.010

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Responses of bacterial community structure in malodorous river sediments to aeration by DGGE

doi: 10.3969/j.issn.1000-5641.2015.02.010

1.
Shanghai Key Lab for Urban Ecological Processes and EcoRestoration, East China Normal University, Shanghai200241, China;
2.
School of Ecological and Environmental Sciences, East China Normal University, Shanghai200241, China

Received Date: 2014-08-07
Publish Date: 2015-03-25

Abstract

Abstract

In this study, 16S rDNA based PCR amplification and denaturing gradientgel electrophoresis (PCRDGGE) as well as sequence alignment from the excised DGGE bands were used to investigate the bacterial diversity and variation. In addition, the redundancy analysis was adopted to assess the relationship between bacterial community structure and environmental factors. The results showed that aeration had marked effects on bacterial community structure in malodorous river sediments and different aeration intensities resulted in variable bacterial community. Amongst them the highest richness of bacterial community was observed with the aeration turbulence of Re=1 810 and DO of 7.35. It was also inferred that moderate aeration facilitated the growths of related bacteria to N and Scycling and theProteobacteria was dominant. It was also found that DO and Re played important roles in bacterial community from river sediments.
- malodorous riversedimentsaerationbacteriaDGGE,

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References(1)

References

[1]

［1］何岩, 沈叔云, 黄民生, 等. 城市黑臭河道底泥内源氮硝化—反硝化作用研究［J］. 生态环境学报, 2012(6):1166 1170.

［2］WU Q, ZHANG R, HUAN S, et al. Effects of bacteria on nitrogenand phosphorus release from river sediment ［J］. Journal of Environmental SciencesChina, 2008, 20(4): 404412.

［3］SANCHEZANDREA I, RODRIGUEZ N, AMILS R, et al. Microbial diversity in anaerobic sediments at río tinto, a naturally acidic environment with a high heavy metal content［J］. Applied and Environmental Microbiology, 2011, 77(17):6085–6093.

［4］HEY, CHENY, ZHANGY, et al. Role of aerated turbulence in the fate of endogenous nitrogen from malodorous river sediments ［J］. Environmental Engineering Science, 2013, 30(1): 1116.

［5］徐欢. 水培植物净化槽对黑臭河水营养盐净化效果及其微生物机制研究［D］. 上海: 华东师范大学, 2012.

［6］HE Y, ZHAO Y C, ZHOU G M, et al. Evaluation of extraction and purification methods for obtaining PCRamplifiable DNA from aged refuse for microbial community analysis［J］. World J Microbiol Biotechnol, 2009, 25:20432051.

［7］MURRAY A E, HOLLIBUGH J T, ORREGO C. Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments［J］. Applied and Environmental Microbiology, 1996, 62(7): 26762680.

［8］ROMANENKOLA, TANAKA N, KALINOVSKAYA NI, et al. Diversity and antimicrobial activity of deepsea surface sediment associated bacteria from the Sea of Japan ［J］.World Journal of and Microbiology and Biotechnology, 2013, 29:11691177.

［9］刘欣. 胶州湾沉积物细菌多样性及菌群时空分布规律研究［D］. 北京:中国科学院大学,2010 .
［10］FUJIOKA R S. Monitoring coastal marine waters for sporeforming bacteria of faecal and soilorigin to determine point from nonpoint source pollution ［J］. Water Science & Technology, 2001, 44(7): 181188.

［11］VELMURUGAN N, KALPANA D, CHO J Y, et al. Phylogenetic analysis of culturable marine bacteria in sediments from South Korean Yellow Sea［J］. Microbiology, 2011, 80 (2), 261272.

［12］RAMOSPADRON E, BORDENAVE S, LIN S, et al. Carbon and sulfur cycling by microbial communities in a gypsumTreated oil sands tailings pond［J］.Environmental Science & Technology, 2010, 45(2): 439446.

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