Ultrasensitive detection of protein biomarker using fluorescent quantum dot probes

WU Si-Min; JIA Chun-Ping; LIU Li-Fen; GAO Wan-Lei; JING Feng-Xiang; Jin-Qing-Hui; ZHANG Hong-Feng; ZHAO Jian-Long

doi:10.3969/j.issn.1000-5641.2016.02.016

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Jul. 2016

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2016 > (2): 144-152

WU Si-Min, JIA Chun-Ping, LIU Li-Fen, GAO Wan-Lei, JING Feng-Xiang, Jin-Qing-Hui, ZHANG Hong-Feng, ZHAO Jian-Long. Ultrasensitive detection of protein biomarker using fluorescent quantum dot probes[J]. Journal of East China Normal University (Natural Sciences), 2016, (2): 144-152. doi: 10.3969/j.issn.1000-5641.2016.02.016

Citation:

WU Si-Min, JIA Chun-Ping, LIU Li-Fen, GAO Wan-Lei, JING Feng-Xiang, Jin-Qing-Hui, ZHANG Hong-Feng, ZHAO Jian-Long. Ultrasensitive detection of protein biomarker using fluorescent quantum dot probes[J]. Journal of East China Normal University (Natural Sciences), 2016, (2): 144-152. doi: 10.3969/j.issn.1000-5641.2016.02.016

Citation:

WU Si-Min, JIA Chun-Ping, LIU Li-Fen, GAO Wan-Lei, JING Feng-Xiang, Jin-Qing-Hui, ZHANG Hong-Feng, ZHAO Jian-Long. Ultrasensitive detection of protein biomarker using fluorescent quantum dot probes[J]. Journal of East China Normal University (Natural Sciences), 2016, (2): 144-152. doi: 10.3969/j.issn.1000-5641.2016.02.016

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Ultrasensitive detection of protein biomarker using fluorescent quantum dot probes

doi: 10.3969/j.issn.1000-5641.2016.02.016

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Author Bio:
吴思敏，女，硕士研究生，研究方向为肿瘤生物学. E-mail: wusimin_mail@163.com.
Received Date: 2015-03-23
Publish Date: 2016-03-25

Abstract

Abstract

In this study, based on the unique properties of quantum dots (QDs) and immune magnetic beads, an fluorescent assay was built to detect low concentration carcinoembryonic antigen (CEA). The assay was carried out in a sandwich formation in which two antibodies simultaneously recognized two separate binding sites on the same target CEA. After enrichment with magnetic separator, the fluorescent intensities produced by the QDs, which were confined to the surface of the beads, could be read efficiently to quantify the concentration of the CEA. For this assay, the limit of detection was 38 pg/mL with the linear range was from 0.39 to 50 ng/mL, and it has been proved with high accuracy and repeatability in tumor marker control. With the high sensitivity, specificity and low sample consumption, this method takes a big step to the early diagnosis of diseases.
- quantum dotsimmunofluorescence assaymagnetic beadstumor marker,

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References(1)

References

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