Determination of sulfonamides by ultra-high performance liquid chromatography with microextraction

LI Yi; ZHANG Yan; ZHANG Ya-ting; HE Pin-gang; WANG Qing-jiang

doi:10.3969/j.issn.1000-5641.2019.02.017

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Mar. 2019

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2019 > (2): 156-163

LI Yi, ZHANG Yan, ZHANG Ya-ting, HE Pin-gang, WANG Qing-jiang. Determination of sulfonamides by ultra-high performance liquid chromatography with microextraction[J]. Journal of East China Normal University (Natural Sciences), 2019, (2): 156-163. doi: 10.3969/j.issn.1000-5641.2019.02.017

Citation:

LI Yi, ZHANG Yan, ZHANG Ya-ting, HE Pin-gang, WANG Qing-jiang. Determination of sulfonamides by ultra-high performance liquid chromatography with microextraction[J]. Journal of East China Normal University (Natural Sciences), 2019, (2): 156-163. doi: 10.3969/j.issn.1000-5641.2019.02.017

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Determination of sulfonamides by ultra-high performance liquid chromatography with microextraction

doi: 10.3969/j.issn.1000-5641.2019.02.017

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

Received Date: 2017-12-04
Publish Date: 2019-03-25

Abstract

Abstract

In this study, the development of an analytical method for the determination of chicken meat based on three-phase hollow fiber membrane microextraction ultra-high performance liquid chromatography (HF-LPME-UHPLC) was explored. A three-phase hollow fiber membrane microextraction system was developed to optimize the extraction conditions of HF-LPME:the extractant was n-octanol, the concentration of NaOH in the receiving phase was 200 mmol/L, the concentration of hydrochloric acid in the sample solution was 1.0 mmol/L, the concentration of Na₂SO₄ was 375 g/L, the extraction temperature was 30°, the stirring rate was 300 r/min, and the extraction time was 4 hours. Chicken meat samples were extracted and analyzed by UHPLC. Chromatographic analysis showed that the six sulfonamides reached baseline separation within six minutes. The linearity of the six sulfonamides in the mass range was good with a correlation coefficient of R² > 0.998 9, the enrichment factors ranged from 188 to 950 times, and the detection limit was 0.03~0.1 ng/mL (S/N=3). The recoveries of sulfonamides flagged in chicken were 85.2%~95.6% with relative standard deviations of 3.13%~4.33% (n=5) at a concentration of 50 μg/kg. The studied method is highly sensitive and reproducible, and can be used for the detection of sulfonamides in chicken.

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References(15)

References

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