Effect of sulfide on the denitrification potential of sediment in black-odor rivers

WANG Shan; ZHU Jin; HE Yan; HUANG Min-sheng; ZHOU Yun-chang

doi:10.3969/j.issn.1000-5641.2019.04.015

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Jul. 2019

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2019 > (4): 156-164

WANG Shan, ZHU Jin, HE Yan, HUANG Min-sheng, ZHOU Yun-chang. Effect of sulfide on the denitrification potential of sediment in black-odor rivers[J]. Journal of East China Normal University (Natural Sciences), 2019, (4): 156-164. doi: 10.3969/j.issn.1000-5641.2019.04.015

Citation:

WANG Shan, ZHU Jin, HE Yan, HUANG Min-sheng, ZHOU Yun-chang. Effect of sulfide on the denitrification potential of sediment in black-odor rivers[J]. Journal of East China Normal University (Natural Sciences), 2019, (4): 156-164. doi: 10.3969/j.issn.1000-5641.2019.04.015

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Effect of sulfide on the denitrification potential of sediment in black-odor rivers

doi: 10.3969/j.issn.1000-5641.2019.04.015

WANG Shan^{1,2
,},
ZHU Jin^1,2,
HE Yan^{1,2
,
,},
HUANG Min-sheng^1,2,
ZHOU Yun-chang^1,2

1.
School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China
2.
Shanghai Key Laboratory of Urbanization Ecological Restoration, East China Normal University, Shanghai 200241, China

Received Date: 2018-07-07
Publish Date: 2019-07-25

Abstract

Abstract

This study aimed to explore the effect of sulfide on sediment denitrification in different concentrations, analyze the response of bacteria, including denitrifying and sulfate-reducing bacteria, and provide a theoretical basis and technical support for improving the denitrification process of sediment in black-odor rivers. The results showed that a low concentration of sulfides (8 mg·L^-1) didn't have a significant effect. In constrast, a moderate concentration of sulfides (40 mg·L^-1, 64 mg·L^-1) promoted the denitrification process; in this range the higher the concentration of sulfides, the faster the rate of nitrate degradation. When the sulfide concentration rises to 96 mg·L^-1 and above, reductive sulfur inhibits denitrification, and the higher the concentration, the more obvious the inhibition. After a period of denitrification of the culture, the bacterial diversity is mainly composed of Proteobacteria, Chlofloflexi, and Bacillus. As the same time, the total number of bacteria in the sediment increased; the abundance ratio of nirS to 16S rRNA gene and dsrB to 16S rRNA gene were 1.42% and 0.05%, respectively. Compared with the original sediment (0.15%, 0.19%), the denitrifying bacteria increased significantly, but the number of sulfate-reducing bacteria decreased.
- black-odor rivers,
- reductive sulfur,
- denitrification,
- sediment

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References(45)

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