In order to study the antioxidation effects of grape seed procyanidins extract (GPE), seven chemiluminescence systems were used to determine the protective effect of GPE on DNA damage caused by ·OH and the scavenging effect of GPE on O2-、OH、H2O2 and ONOO- , and various reactive oxygen species produced during "respiration burst" of whole blood phagocytes. The inhibiting effect of GPE on lipid peroxidation was evaluated by using the colorimetric system of lipoprotein polyunsaturated fatty acids peroxidation induced by Fe2＋. The experiments showed that GPE can effectively scavenge O2-、OH、H2O2 ,ONOO- and various reactive oxygen species prouduced during "respiration burst" of whole blood phagocytes, and inhibit lipid peroxidation (IC50s are 0.2ug/ml、90ug/ml、0.5ug/ml、10ug/ml、130ug/ml、70ug/ml respectively); the inhibiting rate of DNA oxidative damage is approximately 70%, when GPE at a concentration of 25ug/ml is given. These results indicate that GPE os an efficient antioxidant and can effectively scavenge various free radicals and provide effective protection anfinst DNA damage caused by ·OH.