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HUANG Lin-ling, YE Hong, CHEN Shu-de, ZHONG Jian-jiang. Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (4): 16-22.
Citation:
HUANG Lin-ling, YE Hong, CHEN Shu-de, ZHONG Jian-jiang. Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (4): 16-22.
HUANG Lin-ling, YE Hong, CHEN Shu-de, ZHONG Jian-jiang. Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (4): 16-22.
Citation:
HUANG Lin-ling, YE Hong, CHEN Shu-de, ZHONG Jian-jiang. Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (4): 16-22.
Key Laboratory of Optical and Magnetic Resonance Spectroscopy, Minsitry of Education, Department of Physics, East China Normal University,Shanghai 200062,China;
2.
State Key Laboratory of Bioreactor Engineering, East ChinaUniversity of Science and Technology, Shanghai 200237, China
Two dimensional gel electrophoresis(2-DE) was used to analyse changes of the protein expression of suspension cultures of Taxus chinensis 48 hours after pulsed electric field (PEF) elicitation. The 2-DE experiments were repeated for 3 times, and proved to be reliable according to the correlation coefficient analysis. Distinct changes were found from comparison of the protein patterns of control and PEF treated cells. Matrixassisted laser desorption/ionization mass spectrometry and electrospray ionization mass spectrometry were used to analyse some proteins. The results indicated that heterogeneous nuclear ribonucleoprotein(hnRNP), aldose l-epimerase family protein and Ferredoxin-NADP(+) reductase, putative/adrenodoxin reductase are related to the induction of PEF.
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