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ZUO Yi, HUANG Jing, BIAN Hui-fang, YU Zheng-yan, WU Zi-rong. Cloning and Expression of rhGLP-1 Multimers in E.coli(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (4): 110-114,.
Citation:
ZUO Yi, HUANG Jing, BIAN Hui-fang, YU Zheng-yan, WU Zi-rong. Cloning and Expression of rhGLP-1 Multimers in E.coli(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (4): 110-114,.
ZUO Yi, HUANG Jing, BIAN Hui-fang, YU Zheng-yan, WU Zi-rong. Cloning and Expression of rhGLP-1 Multimers in E.coli(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (4): 110-114,.
Citation:
ZUO Yi, HUANG Jing, BIAN Hui-fang, YU Zheng-yan, WU Zi-rong. Cloning and Expression of rhGLP-1 Multimers in E.coli(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (4): 110-114,.
To get a large quantity of human glucagon-like peptide-1, different hGLP-1 multimers containing different copies of rhGLP-1 were constructed. The synthetic hGLP-1 cDNA gene was inserted into pET-32a(+) to get the recombinant plasmid pET32-GLP-1, which could express a fusion protein including thioredoxin, hexahistidine, and rhGLP-1. The coding sequence for the fusion protein was repeatedly inserted into the vector in tandem to get pET32-GLP-1-2 and pET32-GLP-1-3 with two or three copies, respectively. Three different plasmids were transformed into E.coli BLR (DE3) and induced by IPTG. The results demonstrated that the three kinds of E.colicells expressed the desired protein and the percentages of the desired protein increased with the numbers of gene copy. The purified rhGLP-1 showed obvious biological activity of lowering plasma glucose.