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XU Ping, LI Xiao-fang, ZENG Wei-jun, WANG Shui-ping. Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (2): 98-104,.
Citation:
XU Ping, LI Xiao-fang, ZENG Wei-jun, WANG Shui-ping. Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (2): 98-104,.
XU Ping, LI Xiao-fang, ZENG Wei-jun, WANG Shui-ping. Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (2): 98-104,.
Citation:
XU Ping, LI Xiao-fang, ZENG Wei-jun, WANG Shui-ping. Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2006, (2): 98-104,.
Gene expression of VHA-c was detected in Arabidopsis silique, flower, leaf, bolt, root. However, the expression level of VHA-c3 was much higher in leaves than in the other tissues. Analysis of the putative promoter of VHA-c3 was carried out in tobacoo leave system. A series of the promoter mutants were constructed in which various extents of VHA-c3 promoter were placed upstream of the GUS gene in vector pC1305.1. The expression of GUS was analyzed by Agrobacterium-mediated transient expression system.It was found that a 2 812 bp promoter region has high gene expression capacity. And there is a high potential key cis-element for leaf-specific expression between 2812-2 234 bp region.
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