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LIU Yong-qiang, NI Xiao-hua, ZHOU Dan, WANG Xin, GUO Wei-wei. Primary Study of CREB4 Translocation Mechanism from Cytoplasm to Nucleus(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2007, (2): 104-110.
Citation:
LIU Yong-qiang, NI Xiao-hua, ZHOU Dan, WANG Xin, GUO Wei-wei. Primary Study of CREB4 Translocation Mechanism from Cytoplasm to Nucleus(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2007, (2): 104-110.
LIU Yong-qiang, NI Xiao-hua, ZHOU Dan, WANG Xin, GUO Wei-wei. Primary Study of CREB4 Translocation Mechanism from Cytoplasm to Nucleus(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2007, (2): 104-110.
Citation:
LIU Yong-qiang, NI Xiao-hua, ZHOU Dan, WANG Xin, GUO Wei-wei. Primary Study of CREB4 Translocation Mechanism from Cytoplasm to Nucleus(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2007, (2): 104-110.
cAMP response element-binding(CREB) proteins are a family of mammalian transcription activators that mediate cAMP and calcium-dependent gene expression through the cAMP response element (CRE). CREB4 is a novel member of the human CREB family. Yeast two-hybrid system revealed that karyopherinα2 may associate with CREB4215-279aa. Subcellular location of CREB4 and CREB41-279aa showed that the GFP-CREB4 fusion protein was localized in cytoplasm, whereas the fusion protein of GFP-CREB41-279aa was translocated in nucleus. Our experiments further showed that CREB4 and karyopherinα2 were co-located in cytoplasm,but CREB41-279aa and karyopherinα2 were co-located in nucleus. These results suggested that first C-terminal was cleaved and then CREB4was carried into nucleus by karyopherinα2 to activate gene transcription.