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HAN Yi, CHEN Ying-ying, HOU Lian-sheng. Influence of gp150 Protein on Cell Membrane Proteinsduring Dictyostelium Development(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2007, (4): 101-106.
Citation:
HAN Yi, CHEN Ying-ying, HOU Lian-sheng. Influence of gp150 Protein on Cell Membrane Proteinsduring Dictyostelium Development(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2007, (4): 101-106.
HAN Yi, CHEN Ying-ying, HOU Lian-sheng. Influence of gp150 Protein on Cell Membrane Proteinsduring Dictyostelium Development(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2007, (4): 101-106.
Citation:
HAN Yi, CHEN Ying-ying, HOU Lian-sheng. Influence of gp150 Protein on Cell Membrane Proteinsduring Dictyostelium Development(Chinese)[J]. Journal of East China Normal University (Natural Sciences), 2007, (4): 101-106.
To study the change of membrane proteins during Dictyostelium discoideum development, we used wild-type cells KAx-3 and mutant cells AK127. Membrane proteins was prepared by biochemical extraction by TritonX-114, separated using SDS-PAGE and scanned by BIO-PROFILE of VILBER LOURMAT. The results showed that 68 kD,60 kD,54 kD,50 kD,37 kD,34 kD and 31 kD are more stable during wild-type cells KAx-3 and mutant cells AK127 developments; they were not changed by AK127 cells devoid of gp150 proteins developments. The difference of protein content exist in 45 kD, 25 kD, 20 kD and 17 kD proteins, contents of those proteins being increased in the wild cells, and two new 10~12 kD proteins appeared in wild cells after 12 h development. This result indicates contents of those proteins expression are influenced by gp150 protein.