To evaluate the preservation effects of different solutions, viability rate and density were assessed in of Eriocheir sinensis preserved in six solutions (In Vitro) at 4 ℃. Meanwhile viability rate of sperms was assessed by eosin stain. Those six preservation solutions were calcium-free artificial sea water I (Ca2+-FASW I), calcium-free artificial sea water II (Ca2+-FASW II), isostonie sodium chloride (NaCl) solution, aseptic physiological saline, isostonie potassium chloride (KCl) solution and isostonie glucose solution. Results showed that most of sperms preserved in KCl solution and glucose solution were thorough dead after 2 days. While sperms in Ca 2+-FASW I, II still keep alive.Especially in the Ca2+-FASW II, 92.19% sperms survived after 6 days′ preservation. Therefore, Ca2+-FASW II is an excellent solution to preserve Eriocheir sinensis sperms (In Vitro). Furthermore, Ca2+-FASW II was used to study its preservation effect with different sperms destiny, pH value and osmotic pressure. It indicated that no less than 95% sperms survived after 2 days′ preservation at 106~107 sperm/mL at 4 ℃. At least 90% survived after 15 days′ preservation at pH 7~10. More than 75% survived after 15 days′ preservation at 700~850 mOsmol/kg H2O among 10 scalar solutions at the different osmotic pressures (500~1 000 mOsmol/kg H2O) modulated by NaCl. It is concluded that with sperm density at 106~107 sperm/mL, osmotic pressure at 750~850 mOsmol/kg H2O and pH at 7~10, Eriocheir sinensis sperms are preserved in the optimum condition (In Vitro).